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Voltage clamping single cells in intact malpighian tubules of mosquitoes.

Principal cells of the Malpighian tubule of the yellow fever mosquito were studied with the methods of two-electrode voltage clamp (TEVC). Intracellular voltage (V(pc)) was -86.7 mV, and input resistance (R(pc)) was 388.5 kOmega (n = 49 cells). In six cells, Ba(2+) (15 mM) had negligible effects on V(pc), but it increased R(pc) from 325.3 to 684.5 kOmega (P < 0.001). In the presence of Ba(2+), leucokinin-VIII (1 microM) increased V(pc) to -101.8 mV (P < 0.001) and reduced R(pc) to 340.2 kOmega (P < 0.002). Circuit analysis yields the following: basolateral membrane resistance, 652. 0 kOmega; a…

Primary iliopsoas abscess successfully treated by ultrasonographically guided percutaneous drainage.

We report a case of primary iliopsoas abscess successfully treated by ultrasonographically guided percutaneous drainage. A 56-year-old man presented at our hospital with lumbago, right-sided back pain, fever (temperature 38.5 degrees C) and chills. On physical examination, we found dark red skin, swelling, and tenderness localized at the right side at the back of his waist. Laboratory examination showed leukocytosis (white blood cell count 9700/mm3) with a leftward shift and elevated C-reactive protein (5.2 mg/dl). Ultrasonography (US), computed tomography (CT), and magnetic resonance imaging

Переносные микрофлюиды-лаМП для быстрого обнаружения на месте восьми высокопатогенных вирусов.

В последнее время глобальное распространение высокопатогенных вирусов, таких, как вирусы Mpox и Эбола, подчеркивает настоятельную потребность в технологиях обнаружения мультиплексов. Нынешние методы не располагают возможностями для одновременной идентификации, что затрудняет борьбу со вспышками в условиях ограниченности ресурсов и усилия по глобальному наблюдению. Традиционные методы, такие, как количественная цепная реакция полимеразы в режиме реального времени (qPCR), часто непрактичны в районах с ограниченными ресурсами из-за зависимости от лабораторий и специальных кадровых потребностей. Д

Совпадение в аннотации оригинала
BACKGROUND: The recent global spread of highly pathogenic viruses, such as Mpox and Ebola viruses, highlights the urgent need for multiplex … uses: Ebola virus (EBOV), Marburg virus (MARV), Rift Valley fever virus (RVFV), Lassa virus (LASV), yellow fever virus (YFV), Congo Basin Mpox virus (C-MPXV), West African Mpox virus (W-MPXV), and variola virus (VARV), all within a span of 70 min. The assay demonstrated a sensitivity of 7.02 × 102-8 × 104 copies/mL across different targets using simulated clin …

Dengue Virus-2 Infection Affects Fecundity and Elicits Specific Transcriptional Changes in the Ovaries of Aedes aegypti Mosquitoes.

Dengue fever (DF), caused by the dengue virus (DENV), is the most burdensome arboviral disease in the world, with an estimated 400 million infections each year. The Aedes aegypti mosquito is the main vector of DENV and transmits several other human pathogens, including Zika, yellow fever, and chikungunya viruses. Previous studies have shown that the pathogen infection of mosquitoes can alter reproductive fitness, revealing specific vector-pathogen interactions that are key determinants of vector competence. However, only a handful of studies have examined the effect of DENV infection in A. aeg…

Study protocol for the multicentre cohorts of Zika virus infection in pregnant women, infants, and acute clinical cases in Latin America and the Caribbean: the ZIKAlliance consortium.

BACKGROUND: The European Commission (EC) Horizon 2020 (H2020)-funded ZIKAlliance Consortium designed a multicentre study including pregnant … will be collected, and tested for ZIKV and other relevant arboviral diseases (dengue, chikungunya, yellow fever) using RT-PCR or serological methods. A virtual, decentralized biobank will be created. Reciprocal clinical monitoring has been established between partner sites. Substudies of ZIKV seroprevalence, transmission clustering, disabilities and health ec …

Prevalence of chronic comorbidities in dengue fever and West Nile virus: A systematic review and meta-analysis.

BACKGROUND: Flavivirus diseases such as dengue fever (DENV), West Nile virus (WNV), Zika and yellow fever represent a substantial global public health concern. Preexisting chronic conditions such as cardiovascular diseases, diabetes, obesity, and asthma were thought to predict risk of progression to severe infections. OBJECTIVE: We aimed to quantify the frequency of chronic comorbidities in flavivirus diseases to provide an estimate for their prevalence in severe and non-severe infections and examine whether chronic diseases contribute to the increased risk of severe viral expression. METHOD…

Entomological surveillance in active dengue transmission areas of Santo Domingo de los Tsáchilas - Ecuador: Epidemiological importance of Aedes aegypti and the recent establishment of Aedes albopictus.

Dengue virus (DENV) remains a significant public health concern in Ecuador, with Aedes aegypti as the primary vector. In recent years, the d … rate of 33.69 %. No Dengue Virus 3 (DENV-3), Dengue Virus 4 (DENV-4) serotypes, Zika, chikungunya, yellow fever, or West Nile virus were detected. Spatial and entomological risk analyses identified high-risk localities, particularly in urban parishes such as Río Verde and Santo Domingo, where both serotypes were co-circulating. Aedes albopictus was present in …

Preparation and application of YFV-17D-derived Anterograde Trans-neuronal Viral Vectors for Neuroscience Research.

Trans-neuronal viruses that spread between synaptically connected neurons have become invaluable tools in neuroscience, enabling circuit mapping and targeted delivery of genetic material to cells within defined pathways. Although most available trans-neuronal viral tracers propagate retrogradely, an anterograde trans-neuronal virus that spread from presynaptic to postsynaptic neurons would substantially expand experimental capabilities. We previously demonstrated that YFV-17D-a live attenuated yellow fever vaccine used clinically for decades-spreads anterogradely along neuronal circuits. We fu…

Highly Sensitive Si-Based Electrolyte-Gated Transistor Array for Multiplexed Detection of Arboviruses.

Multiplexed detection of arboviruses using a 4 × 4 Si-based electrolyte-gated transistor (EGT) array functionalized with specific aptamers has been investigated. The Si-based EGTs were fabricated using conventional Si microfabrication processes. The EGTs showed excellent intrinsic electrical characteristics, including a low threshold voltage of 0.8 V, a sub-threshold swing of 75 mV/dec, and a gate leakage of <10 pA, ensuring uniform device performance with low device-to-device variation. Aptamers specific to the yellow fever virus nonstructural protein 1 (YF), dengue virus nonstructural protei…

Outbreaks and incidence of vector-borne diseases in Colombia (2007-2024): Impact of climate change and deforestation.

INTRODUCTION: Vector-borne diseases pose a public health challenge in Colombia, influenced by climatic and environmental factors. El Niño and deforestation can alter vector habitats, affecting the incidence of dengue, Zika, chikungunya, malaria, cutaneous leishmaniasis, and yellow fever. OBJECTIVE: This study analyzes the relationship between these variables and vector-borne diseases incidence in Colombia (2007-2024). MATERIALS AND METHODS: An ecological study was conducted using incidence and outbreak data for six vector-borne diseases, linked to climate information, El Niño, and deforestat…

Time-series analysis of transcriptomic changes due to permethrin exposure reveals that Aedes aegypti undergoes detoxification metabolism over 24 h.

Insecticide resistance is a multifaceted response and an issue across taxa. Aedes aegypti, the mosquito that vectors Zika, dengue, chikungunya, and yellow fever, demonstrates high levels of pyrethroid resistance across the globe, presenting a challenge to public health officials. To examine the transcriptomic shifts across time after exposure to permethrin, a 3'Tag-Seq analysis was employed on samples 6, 10, and 24 h after exposure along with controls. Differential expression analysis revealed significant shifts in detoxifying enzymes and various energy-producing metabolic processes. These fin…

TRIM22 suppresses Zika virus replication by targeting NS1 and NS3 for proteasomal degradation.

BACKGROUND: Recognition of viral invasion by innate antiviral immune system triggers activation of the type I interferon (IFN-I) and proinfl … we found that TRIM22 also inhibited other flaviviruses infection including dengue virus (DENV) and yellow fever virus (YFV). CONCLUSION: Thus, TRIM22 is an ISG with important role in host defense against flaviviruses through binding and degradation of the NS1 and NS3 proteins.

Вакцинация с помощью домена III вируса Зика вызывает нейтрализацию антител и частичную защиту от азиатского генотипа в иммунокомпетентных мышах.

Вирус Зика (ZIKV) — пахотный флавивирус, относящийся к семейству флавивирид, например, денге (DENV), желтая лихорадка и вирус Западного Нила. Вспышка инфекции ZIKV может создать серьезную угрозу для здоровья населения, поскольку эта инфекция непредсказуема и вызывает сильную патологию, такую, как синдром Гильан-Барре и неонатальная микроцефалия. Однако санкционированной вакцины ZIKV пока еще нет, в то время как несколько кандидатов на вакцины находятся в стадии разработки. В этом исследовании мы создали рекомбинированную вакцину ZIKV (Z_EDIII), которая включает в себя белковую домену ZIKV III

Совпадение в аннотации оригинала
BACKGROUND: Zika virus (ZIKV) is a mosquito-borne flavivirus classified in Flaviviridae family such as dengue (DENV), yellow fever, and West Nile virus. An outbreak of ZIKV infection can pose a major public health risk because the contagion is unpredictable and induces severe pathology such as Guillan-Barre syndrome and neonatal microcephaly. However, an aut…

Investigation of immune response induction by Japanese encephalitis live-attenuated and chimeric vaccines in mice.

The Japanese encephalitis (JE) live-attenuated vaccine SA14-14-2 and the chimeric vaccine IMOJEV (JE-CV) are two kinds of vaccines available for use worldwide. JE-CV was previously known as ChimeriVax-JE, that consists of yellow fever vaccine 17D (YFV-17D) from which the structural genes (prM/E) have been replaced with those of SA14-14-2. This study aimed to investigate the neutralizing antibody, protection efficacy, and specific T-cell response elicited by both vaccines in mice. The neutralizing antibodies produced by JE-CV were slightly lower than those produced by SA14-14-2, but the protect…

Regulatory Role of Host MicroRNAs in Flaviviruses Infection.

MicroRNAs (miRNAs) are small non-coding RNA that affect mRNA abundance or translation efficiency by binding to the 3'UTR of the mRNA of the target gene, thereby participating in multiple biological processes, including viral infection. Flavivirus genus consists of small, positive-stranded, single-stranded RNA viruses transmitted by arthropods, especially mosquitoes and ticks. The genus contains several globally significant human/animal pathogens, such as Dengue virus, Japanese encephalitis virus, West Nile virus, Zika virus, Yellow fever virus, Tick-borne encephalitis virus, and Tembusu virus.…

Multimodal Integration Across Spatiotemporal Scales to Guide Invertebrate Locomotion.

Locomotion is a hallmark of organisms which has enabled adaptive radiation to an extraordinarily diverse class of ecological niches, and all … dal integration in genetic model systems, including the fruit fly, Drosophila melanogaster, and the yellow fever mosquito, Aedes aegypti. We identify that summation (e.g., gating) and weighting-which are inherent computations of spiking neurons-underlie multimodal integration across spatial and temporal scales, therefore suggesting collective strategies to gui …