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Presence of Anisakis pegreffii in farmed sea bass (Dicentrarchus labrax L.) commercialized in Southern Italy: A first report.

We examined 151 European sea bass (Dicentrarchus labrax L.) samples from farms and fish markets of Sicily (Southern Italy) for Anisakidae larvae detection. All the samples were examined by visual inspection and modified chloro-peptic digestion. Two nematode larvae were found in the viscera of only one European sea bass sample from a farm located in Greece (FAO 37.3), giving a total prevalence of infestation of 0.7%. No other parasites were found after chloro-peptic digestion of the samples. The larvae were morphologically ascribed, at genus level, to morphotypes I and molecularly identified as…

Prevalence of Muscular Sarcosporidiosis in Slaughtered Domestic Pigs in Perak, Peninsular Malaysia.

Sarcosporidiosis is a disease caused by intracellular protozoan parasites, namely, Sarcocystis spp. In pigs, three species of Sarcocystis spp. have been recognised, including Sarcocystis meischeriana, Sarcocystis porcifelis and Sarcocystis suihominis. The aim of this study is to determine the prevalence of muscular sarcosporidiosis in pigs using the pepsin digestion technique. A total of 150 fresh heart, oesophagus and thigh muscle samples from 50 Yorkshire and Landrace pigs were collected from two local abattoirs in Perak from May to August 2014. All the fresh muscle samples were thoroughly e…

Haematic silicon in drowning.

The aim of this paper was to evaluate silicon (Si) concentration in human whole ventricular blood as a further potential chemical marker in the diagnosis of drowning. We employed an acidic digestion for the extraction of soluble Si, and an alkaline digestion for the determination of total Si, including particulate matter, both arising from drowning medium. 29 suspected drowning situations, 24 in fresh water (Fw) and 5 in seawater (Sw), were examined. The difference in Si concentration between the left and right ventricular blood (Si ΔL-R) was measured and alkaline Si ΔL-R seems, indeed, a pote…

Chitinases in Pneumocystis carinii pneumonia.

Pneumocystis pneumonia remains an important complication of immune suppression. The cell wall of Pneumocystis has been demonstrated to poten … le homolog to the fungal chitin biosynthesis protein Chs5. We also observed the impact of chitinase digestion on Pneumocystis-induced host inflammatory responses by measuring TNFα release and mammalian chitinase expression by cultured lung epithelial and macrophage cells stimulated with Pneumocystis cell wall isolates in the presence and absence of exogenou …

Hemagglutinin-33 of type A botulinum neurotoxin complex binds with synaptotagmin II.

Botulinum neurotoxin type A (BoNT/A), the most toxic substance known to mankind, is produced by Clostridium botulinum type A as a complex with a group of neurotoxin-associated proteins (NAPs) through polycistronic expression of a clustered group of genes. NAPs are known to protect BoNT against adverse environmental conditions and proteolytic digestion. Hemagglutinin-33 (Hn-33) is a 33 kDa subcomponent of NAPs that is resistant to protease digestion, a feature likely to be involved in the protection of the botulinum neurotoxin from proteolysis. However, it is not known whether Hn-33 plays any r…

Determination of light chain restriction in fine-needle aspiration-type preparations of B-cell lymphomas by mRNA in situ hybridization.

The purpose of this study was to investigate whether B-cell clonality could be demonstrated in fine-needle aspiration (FNA)-type preparations by using automated and manual in situ hybridization (ISH) systems. FNA-like preparations were made from 10 cases of B-cell lymphoma and 5 cases of reactive lymphoid hyperplasia. Kappa/lambda expression was determined using an automated mRNA ISH assay or a manual ISH system. Other variables tested included type and length of fixation, protease digestion, and time in chromogen. Clonality data were corroborated by either flow cytometry or tissue-based analy…

Sludge thermal oxidation processes: mineral recycling, energy impact, and greenhouse effect gases release.

Different treatment routes have been studied for a mixed sludge: the conventional agricultural use is compared with the thermal oxidation processes, including incineration (in gaseous phase) and wet air oxidation (in liquid phase). The interest of a sludge digestion prior to the final treatment has been also considered according to the two major criteria, which are the fossil energy utilisation and the greenhouse effect gases (CO2, CH4, N2O) release. Thermal energy has to be recovered on thermal processes to make these processes environmentally friendly, otherwise their main interest is to ext…

Simultaneous detection of malignant hyperthermia and genetic predisposition for improved litter size in pigs by multiplex PCR-RFLP.

The ryanodine receptor gene (RYR1) and the estrogen receptor gene (ESR) are the best commercially used markers for predisposition of stress susceptibility (malignant hyperthermia--MH) and increased litter size, respectively. A simplified method of simultaneous detection of MH and ESR genotypes has been developed. The method is based on simultaneous amplification of fragments of two genes by multiplex PCR and subsequent digestion of the products with two restriction enzymes. The PCR and the digestion could be performed in a single tube and all genotypes could be detected by electrophoretic sepa…

Comparison of serotyping, ribotyping and pulsed-field gel electrophoresis for distinguishing group A Streptococcus strains isolated in Albania.

Conventional serotyping for T antigens, rRNA gene restriction fragment length polymorphism analysis (ribotyping) and pulsed-field gel electrophoresis were compared for distinguishing among group A streptococci isolated in Albania between 1980-1982 and in 1995. A total of twelve serotypes were identified among seventy GAS strains. Ribotyping revealed eight and eleven distinct patterns after digestion with HindIII and PvuII, respectively. Twenty-three strains of serotype T12 were subdivided in 10 ribotypes and 11 strains of T2 serotype were differentiated in 5 ribotypes. By comparison, PFGE gene…

Detection and species identification of mycobacteria in paraffin sections of lung biopsy specimens by the polymerase chain reaction.

The authors analyzed 25 paraffin-embedded lung biopsy specimens for mycobacterial DNA by the polymerase chain reaction (PCR) from patients with pulmonary mycobacterial infection demonstrated by acid-fast stain, culture, or both. DNA was extracted from 4 microM unstained paraffin sections by proteinase K digestion followed by freeze-fracturing and amplified by nested PCR with primers for the mycobacterial 65-kDa antigen gene. Mycobacterial DNA was detected in 7 of 7 wedge and 9 of 18 transbronchial biopsy specimens by PCR. Nested PCR with direct visualization on an agarose gel was as sensitive …

Chromatin organization in Entamoeba histolytica.

The chromatin structure of Entamoeba histolytica was investigated. It was found that this protozoan organizes its chromatin in nucleosome-like particles 10 nm in diameter, but digestion of the chromatin with micrococcal nuclease did not render a regularly spaced DNA ladder in agarose gels. Southern blot analysis of the products of Entamoeba chromatin digestion using total amebic DNA and a non-transcribed repetitive sequence produced a banding pattern characteristic of eukaryotic chromatin with a repetitive size of approximately 130 bp. Conversely, hybridization with two active gene probes, act…

Comparison of three methods for detection of prolonged experimental trichinellosis in pigs.

Three methods were employed for the diagnosis of porcine trichinellosis. The pooled sample digestion method and trichinoscopy served as European Community (EC) reference techniques, whereas the reliability of the Enzyme Linked Immunosorbent Assay (ELISA) was tested by 11 laboratories of the European Community and Sweden. Three groups of 6 piglets each were orally inoculated with 50, 150 and 1500 Trichinella spiralis larvae into each animal. Another group of 6 animals served as a non-infected control. Animals were slaughtered and serum and muscle samples were collected at Weeks 4, 12 and 40. Th…

[Primary structure of tRNA2Leu of bovine mammary gland. Oligonucleotides of T1-RNAse hydrolysate. Reconstruction of a total nucleotide sequence].

The oligonucleotides obtained by digestion of tRNA2Leu from cow mammary gland with T1 RNase were separated by micro-column chromatography on DEAE-cellulose in 7 M urea at pH 7,5 and 3,7, and in addition on Dowex 1 x 2. The digest consisted of 18 individual components, the larger being a tridecanucleotide. Micro-column chromatography of nucleotides on anion-exchanger AG 1 x 8 and nucleosides on Aminex A-6 was used to determine the base composition of the oligonucleotides. The oligonucleotide structure was established using terminal analysis, hydrolysis by pancreatic and U2-RNases and incomplete…

Идентификация Рикитсии рикетси в формализованных тканях с парафинами по иммунной флюоресценции.

С небольшим модификацией методов пищеварения трипсина Рикетсиа рикеттсии была продемонстрирована конкретно иммунофлюоресцентной пятнистостью в пятнах тканей, покрытых формальным слоем парафина, от человека, резусной обезьяны и подопытной свиньи с пятнами Рокки Маунтин и в инфицированных мембранах из куриного эмбриона. Ткани были вырезаны на 4 микрона и, используя гелтен в качестве клейки для тканей, были обработаны в обычном порядке. После этого секции переваривались в рефрижераторный 0,1% трипсина в течение 16 часов, промывались и пятна специально для R. rickettsii в результате прямой или кос

Совпадение в аннотации оригинала
With slight modification of a trypsin digestion technique, Rickettsia rickettsii were demonstrated specifically by immunofluorescence staining in Formalin-fixed, paraffin-embedded tissue sections from a human, rhesus monkey, and guinea pig with Rocky Mountain spotted fever and in infected membranes from a chicken embryo. Tissues were cut at 4 micron and, usi…

[Detection of Trichinella spiralis infection in spontaneously infected pigs intended for slaughter using the enzyme-linked immunosorbent assay (ELISA) (author's transl)].

In a study of seventy-seven pigs intended for saughter, from two farms on which Trichinella infections had previously been detected by trichinoscopy, the sensitivity of the enzyme-linked immunosorbent assay (ELISA) was compared with that of two direct (trichinoscopy and digestion) and three indirect (serological) methods of detection (fluorescent antibody technique, counter-electrophoresis and Ouchterlony's agar gel diffusion method). In five out of seventy-seven pigs, the digestion procedure revealed the presence of larvae of Trichinella spiralis in the diaphragm. Four of these pigs showed a …

Type-specific antigens of group B type Ic streptococci.

The type-specific antigens of group B type Ic (old designation type Ii) streptococci were extracted, purified, and characterized by serologi … ts of two serologically active determinants, one of which is susceptible to both trypsin and pepsin digestion and the other to pepsin but not to trypsin digestion. Acrylamide gel electrophoresis of the partially purified Ic antigen resulted in the occurrence of both determinants throughout the length of the gel, as shown by double gel diffusion slides.

[Duplication of the alimentary canal in infants and children].

This is a review of 30 duplications of the alimentary tract in 28 patients treated at the Surgical Unit of the Children's Department of the Medical University of Pécs, Hungary and at the Department of Pediatric Surgery of the Medical Academy of Dresden, Germany, from 1964 to 1989. The ages of patients ranged from 1 day to 13 years, 80 per cent were less than 2 years of age at initial presentation. There were 6 thoracic, 20 abdominal and 2 thoracoabdominal duplications. Distended abdomen, vomiting, bowel obstruction and palpable abdominal mass were most frequently encountered. Plain thoracic an

A Suspension-Trapping Protocol for Bottom-Up Proteomics Sample Preparation.

Bottom-up proteomics workflows encompass several key stages, including sample preparation, data acquisition, and data analysis. Of these, sample preparation is the initial and critical stage, as it significantly influences the depth, reproducibility, and reliability of subsequent mass spectrometry-based analyses. While several main digestion strategies exist, including in-gel, in-solution, and filter-aided methods, each presents distinct trade-offs in terms of throughput, contamination removal, and applicability to complex biological matrices. The Suspension Trapping (S-Trap) method offers a c…

Hericium erinaceus β-Glucan Improves Post-Prandial Glycemic Homeostasis in Association With Changes in Akkermansia muciniphila, Serum Metabolites, and Gut Hormones.

Post-prandial glycemia is an important indicator of metabolic health and early glucose dysregulation. Mushroom-derived β-glucans have potential as functional ingredients for glycemic control, but their comparative efficacy and regulatory mechanisms remain unclear. In this study, β-glucans from oyster mushroom (OMBG), Ganoderma lucidum (GBG), and Hericium erinaceus (HEBG) were evaluated using structural characterization, functional assays, simulated digestion, colonic fermentation, and in vivo experiments, with oat β-glucan (OBG) included as a reference. OBG showed strong glucose diffusion-reta…

Bovine Milk Protein-Derived Preparations and Their Hydrolysates as Sources of ACE-Inhibitory, DPP-IV-Inhibitory, and Antioxidative Peptides Analyzed Using in Silico and in Vitro Protocols.

Bovine milk protein preparations (MPPs), namely micellar casein concentrate (MCC), serum protein concentrate (SPC), and MCC with ultrafiltrated buttermilk permeate (MBP), were analyzed as sources of inhibitors of angiotensin-converting enzyme (i.e., ACE) and dipeptidylpeptidase IV (i.e., DPP-IV) as well as antioxidative peptides. The studies involved in silico predictions of the release of biopeptides from bovine milk proteins. Then, all MPPs were subjected to the simulated gastrointestinal digestion using the INFOGEST protocol. Results using a BIOPEP-UWM database tool indicated that 59 biopep…