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Effect of storage time on the stability of selected blood gases, electrolytes, and metabolite concentrations in dairy cattle whole blood and plasma.

Blood gases, electrolytes, and metabolites assist clinicians and researchers with disease diagnosis and prognosis, and can inform treatment decisions on farms. Delays between sample collection and processing can lead to significant changes in these parameters, affecting their clinical and biological value. Therefore, the objective of this study was to evaluate the stability of selected blood gas, electrolyte, and metabolite measurements in dairy cattle whole blood samples stored at 4°C, in addition to frozen plasma samples stored at -80°C for different storage periods. Multiple blood samples w…

Determining Frequency of Multiple Organ System Involvement and Concurrent Lesions Identified in Feedyard Mortalities and Potential Associations with Cattle Demographics.

Necropsies are commonly used to diagnose the causes of death in feedyard cattle, but the documentation of multiple organ system involvement and concurrent lesions is limited. This observational study aimed to determine the frequency of such findings and their associations with animal demographics. Systemic necropsies were conducted for 889 cattle mortalities with minimal autolysis across six feedyards in the Central High Plains during the summers of 2022 and 2023. Lesions and abnormalities were recorded along with arrival weight, sex, days on feed (DOFs), and number of treatments. The results …

New findings of ciliates Buxtonella sulcata in the faeces of cattle from northern regions of Russia and comparative analysis of Buxtonella species.

Buxtonella sulcata is a common ciliate that inhabits the intestines of cattle and can cause diarrhea and other clinical signs of infection. The distribution of B. sulcata in Russia has been practically unstudied, while the buxtonellosis it causes can have a negative impact on the health of cattle. This study aimed to morphologically and molecularly identify cysts and trophozoites of B.sulcata. This study provides data on the first finding of infection with B. sulcata ciliates in cattle from two northern regions of Russia. The structural features of B. sulcata were studied using immunofluoresce…

Knowledge, attitudes, and practices of cattle farmers in Maharashtra provinces of India on tick-borne diseases: insights for sustainable control strategies.

A KAP survey on ticks and tick-borne diseases (TTBDs) among cattle farmers of Maharashtra state, India was conducted. A total of 137 farmers were surveyed with majority aged 41-50, having 6-20 cattle. Education-wise, most of the farmers had high school or college education. Land holding patterns showed majority of farmers have small farms. Farmers preferred high-yielding exotic breeds. Participants observed ticks on livestock, grasses, and forest areas. Ticks were reported in warm and cold regions, affecting cattle productivity. Farmers showed mixed awareness on tick-borne diseases and managem…

Molecular Characterization and Genetic Diversity of Isolated Foot-and-Mouth Disease Viruses Circulating in Cattle in The Mekong Delta Provinces, Vietnam.

Foot-and-mouth disease (FMD) is a highly contagious viral disease of cloven-footed livestock caused by foot-and-mouth disease virus (FMDV). FMD has significant impacts on farmers and national economies. The evolution and mutation of FMDV have contributed to the emergence of new strains of FMDV. Sequences of VP1 from 11 FMDV isolates in the Mekong Delta Provinces were obtained by Sanger sequencing technology. The phylogenetic analysis of VP1 sequence elucidated that 8 FMDV isolates including O-VN-CTU-VL01 (PP897837), O-VN-CTU-VL02 (PP897838), O-VN-CTU-TV01 (PP897840), O-VN-CTU-TV02 (PP897841),

First detection of Crimean Congo Hemorrhagic Fever antibodies in cattle and wildlife of southern continental France: Investigation of explanatory factors.

Crimean-Congo Hemorrhagic Fever (CCHF) is a tick-borne zoonosis of major public health concern, not only because of its potential for severe outcomes in humans, but also due to its endemic presence in many regions and its expanding geographic distribution. We report on the first serological survey conducted in mainland France to detect antibodies against the Crimean-Congo Hemorrhagic Fever Virus (CCHFV) in domestic and wild fauna, and provides critical insights into the virus's circulation. We analyzed 8,609 cattle sera and 2,182 wildlife sera collected across the French Mediterranean region f…

Сопоставление внутреннего анализа иммунофлюоресценции (IFA) и коммерческого анализа иммуносорбента, связанного с ферментом (ELISA), для инфекции рогатого скота Coxiella.

Коксиэллоз, часто асимптоматический у животных, может вызвать значительные репродуктивные потери и массовое сбрасывание хвороста Коксиеллы во время половинки. Серологический надзор имеет важнейшее значение для выявления позитивных стада и осуществления мер контроля в целях защиты животных и здоровья людей. Всемирная организация по охране здоровья животных (ВОАХ) рекомендует провести анализ иммуносорбента, связанного с ферментом (ЭЛИСА), для обследований животных на основе их высокой чувствительности и оперативной простоты. Однако во многих странах отсутствие разрешенных коммерческих комплектов

Совпадение в аннотации оригинала
Coxiellosis, often asymptomatic in animals, can cause significant reproductive losses and massive shedding of Coxiella burnetii during partu … iable alternative for diagnosing and conducting epidemiological surveys of C. burnetii infection in cattle, particularly in settings where no commercial kit is available. Its high specificity and PPV make it especially valuable for confirming infection status in surveillance programs.

Temporal alteration in ovarian follicle fate with hormonal and hemodynamic perspectives during receptive phase of estrous cycle in endometritic cattle.

The present research was carried out to assess the serum progesterone (P4) concentration and uterine hemodynamics at estrus till ovulation in cyclic cows (N = 130) with healthy or diseased uterus. At estrus, 85 cows were diagnosed with clinical endometritis (CE; n = 44) and sub-clinical endometritis (SCE; n = 41); whereas, 45 cows being served as control namely no endometritis (NE; n = 45) were included in the study. Serum progesterone estimation at 12 - 14 and 40 hr after the onset of estrus and Doppler sonography of both middle uterine arteries were done to envisage the uterine hemodynamics

Effect of feeding a dried distillers' grains with solubles diet on the metabolism of the intestinal wall in Guanling crossbred cattle: a preliminary assessment.

Dried distillers' grains with solubles (DDGS)-based diets are nutritious and can improve the inflammations and intestinal immunity in livestock. However, there is limited research examining the effect of feeding DDGS-based diets on changes in intestinal metabolites and related pathways in livestock. In this study, six Guanling crossbred cattle (Guizhou Guanling Yellow cattle × Simmental cattle) were selected and divided into a basal diet (BD) group and an experimental group fed with DDGS replacing 25% of the daily ration concentrates (DDGS) (n=3), respectively. Fresh jejunum (J), ileum (I) and…

Seroprevalence of bovine leukemia virus in cattle and buffaloes in the border provinces of the Eastern Anatolia region, Türkiye: insights into the eradication of infection.

Bovine leukemia virus (BLV) is the causative agent of enzootic bovine leukosis, an oncogenic deltaretrovirus that has emerged as a potential zoonotic infection. The BLV naturally infects cattle and causes economic losses through a slow persistent infection with various clinical symtoms following preleukosis. The main objective of this study was to determine the seroprevalence of BLV antibodies in cattle and buffaloes in the border provinces of the Eastern Anatolia region, Türkiye, using the agar gel immunodiffusion (AGID) assay and enzyme-linked immunosorbent assay (ELISA). For this purpose, a…

The fly route of extended-spectrum-β-lactamase-producing Enterobacteriaceae dissemination in a cattle farm: from the ecosystem to the molecular scale.

INTRODUCTION: This study aimed to understand the origin and to explain the maintenance of extended-spectrum β-lactamase (ESBL) Enterobacteri … SBL-producing strains. Notably, E. coli ST3268 was recurrently isolated from both flies (n = 9) and cattle (n = 5). These E. coli ST3268/bla CTX-M-15/bla TEM-1B have accumulated multiple plasmids and genes, thereby representing a reservoir of resistance and virulence factors. Our findings suggest that flies could act as effective mechanical vectors for a …

Small intestinal incarceration caused by external herniation can be diagnosed clinically in cattle, but laparotomy is required to confirm internal incarceration.

OBJECTIVE: To describe the clinical, laboratory and ultrasonographic findings, treatment, and outcome of cattle with small intestinal incarceration (SII) through internal and external hernias. METHODS: The medical records of 85 cattle with SII admitted between January 1, 1987, and December 31, 2019, were retrospectively reviewed. The long-term outcome was determined 2 years after discharge. RESULTS: 85 cattle had herniation of the small intestine through congenital or acquired openings in mesentery or omentum (internal herniation; n = 60) or the abdominal wall (external herniation; 25). The …

Increasing Beef Production in the Northern Region of the Republic of Kazakhstan Using the Genetic Resources of Aberdeen Angus Cattle of Different Genotypes.

This article presents the findings of a scientific study investigating the efficacy of various assessment techniques used to evaluate the adaptability and productive qualities of Aberdeen Angus cattle on three prominent farms in the northern region of the Republic of Kazakhstan. A comprehensive analysis of the haematological and biochemical parameters of experimental groups of cattle with different genotypes (American, Canadian, and Estonian selection) was conducted. The studies revealed notable variability in haematological and biochemical indicators, contingent on the origin. Concurrently, t…

Replacing rice straw with peanut vine and Broussonetia papyrifera silage in beef cattle feed reduced the use of soybean meal.

The present study investigated whether replacing dietary rice straw with peanut vine (PEV) and Broussonetia papyrifera silage (BPS) reduces the use of soybean meal and explored its effects on the growth performance, blood biochemical indicators, serum metabolomics, and meat quality of fattening bulls. Forty-five Simmental crossbred bulls (initial body weight = 484.29 ± 8.49 kg) were randomly allotted into three dietary treatment groups (n = 15): (1) CON, 5% rice straw (DM basis); (2) PEV, 5% peanut vine (DM basis); and (3) BPS, 5% B. papyrifera silage (DM basis). The remaining roughage for all

Evolution over the last 40 years of the assisted reproduction technologies in cattle - the Brazilian perspective I - timed artificial insemination.

The reproductive efficiency of the herd is correlated with higher productivity in livestock. Reproduction biotechniques, such as ovulation synchronization protocols, are important to optimize production and accelerate genetic profit in beef and dairy herds. The objective of this review is to describe the evolution over the last 40 years of the artificial insemination (AI) and the timed-AI (TAI) protocols in cattle from a Brazilian perspective. TAI protocols are based on synchronizing emergence of the wave of follicular growth, controlling circulating progesterone (P4) concentrations, stimulati…

The influence of corpus luteum and first-wave dominant follicle location on conception rate after artificial insemination in Holstein cattle.

The influence of post-ovulatory corpus luteum (CL) and first-wave dominant follicle (1DF) location on pregnancy establishment in cattle remains controversial. This study aimed to reveal the relationship between CL and 1DF positioning, pregnancy outcomes, and blood steroid hormone levels following artificial insemination (AI) in Holstein cattle. Data from heifers (n = 163) and cows (n = 69) with optimally timed AI, defined as ovulation within 24 h post-AI, were analyzed to determine associations between CL (left/right ovary) and 1DF (ipsilateral/contralateral to CL) locations and conception rat…

Characterization of the Temperament and Reactivity of Nelore Cattle (Bos indicus) Associated with Behavior Scores during Corral Management in the Humid Tropics.

The evaluation of the reactivity and distress of cattle during corral management, by means of subjective scores, aims at the standardization of behavioral indicators, through non-invasive methods, in addition to enabling the development of more appropriate management practices, thus promoting the comfort and well-being of these animals. Therefore, in this study, we aimed to characterize the temperament and distress of cattle managed in a corral using behavioral indicators during the rainiest period. For this, the experiment was conducted on a property located in the municipality of Mojuí dos C…

Investigation of preinguinal approach for removal of urachal abscess in three Japanese black cattle older than 18 months of age.

This study investigated the diagnostic and surgical management of urachal abscessation involving the urinary bladder in three cattle aged >18 months. While the abdominal floor or paralumbar fossa are typically considered for accessing the urachus and bladder in calves and heifers, the distance is too great to perform the procedure under direct vision in adult cows. Therefore, a novel preinguinal approach was used for access in cows with urachal abscesses after 18 months. Access was successfully achieved in all the three cows through a preinguinal incision to the urachal abscess at the apex of …

Bacterial Communities of House Flies from Beef and Dairy Cattle Operations are Diverse and Contain Pathogens of Medical and Veterinary Importance.

Adult house flies (Musca domestica L.) are important reservoirs and mechanical vectors of bacteria in livestock operations. House fly bacterial communities are influenced by their local environment, yet a comprehensive understanding of bacterial diversity, pathogen prevalence, and bacterial source is not fully understood. We characterized bacterial communities from adult female house flies and associated manure samples from beef and dairy cattle farms in Kansas, Oklahoma, and Texas over four months (July-October). Bacterial community composition in flies and manure reflected the local environm…

Evaluation of a Postbiotic on Salmonella enterica Prevalence, Serotype Diversity, and Antimicrobial Resistance in the Subiliac Lymph Nodes of Cull Dairy Cattle.

This study aimed to determine whether the farm-level use of a Saccharomyces cerevisiae-based postbiotic was associated with Salmonella prevalence and concentration, serotype diversity, and antimicrobial resistance in the subiliac lymph nodes (LN) of cull dairy cows. In collaboration with two commercial processing plants in the Southwestern (SW) and Northeastern (NE) regions of the U.S., cull dairy cattle lots processed in the same week from dairy farms that fed or not the postbiotic were sampled. Up to 20 LN were collected from dairy cattle from each supplier farm at least once every season. S…