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Elimination of repeated clot formation in mouse ascitic fluid containing arbovirus antibodies.

Repeated clot formation in mouse ascitic fluids containing antiviral antibody was eliminated by acid precipitation of the fibrinogen.

No-known-vector flaviviruses exhibit diverse replication and virulence phenotypes in mice.

The Orthoflavivirus genus (flaviviruses) includes globally significant arboviruses, which cycle between arthropod vectors (mosquitoes and ti … aviviruses that cause human disease are transmitted by mosquitoes and ticks (e.g., West Nile virus, yellow fever virus, tick-borne encephalitis virus). But there are related flaviviruses that are not known to infect arthropods, so are thought to spread by vector-independent routes (no-known-vector flaviviruses, NKVFVs). Not much is known about NKVFVs, but they …

Integrated In Silico, In Vivo, and Deep Learning Approaches in the Discovery of Novel Candidate Molecules for Aedes aegypti Control.

The mosquito Aedes aegypti is a primary vector responsible for transmitting major arboviruses, including dengue, Zika, chikungunya, and yellow fever. Increasing resistance to conventional synthetic insecticides, combined with their well-known environmental drawbacks, underscores the urgent need for more selective, sustainable, and effective strategies for vector control. Chalcones have been previously identified by our research group as a promising chemical class of larvicidal agents, with preliminary evidence for distinct mechanisms of action. More recently, an additional strategy for integra…

Diffuse Large B-Cell Lymphoma Presenting With Superior Vena Cava Syndrome and Recurrent Pleural Effusions in a Patient With Angelman Syndrome: A Case Report.

Superior vena cava (SVC) syndrome is an uncommon but important presentation of mediastinal malignancy and can rapidly progress to respiratory compromise. We report a case of diffuse large B-cell lymphoma (DLBCL) in a patient with Angelman syndrome and seizure disorder, highlighting the diagnostic and airway challenges encountered during evaluation. A 42-year-old woman with Angelman syndrome presented with progressive neck and facial swelling after two weeks of fever, chills, and rhinorrhea. She reported worsening dyspnea and was admitted for concern for superior vena cava syndrome. Chest imagi

Аналитическая и клиническая оценка группы СТАНДАРД M10 Arbovirus для обнаружения, серотипирования и многоразового скрининга в Северной и Южной Америке.

Фон/цели: Арбовирусы, включая вирус денге (DENV), вирус Зика (ZIKV), вирус Чикунгуньи (CHIKV), вирус желтой лихорадки (YFV) и вирус Западного Нила (WNV), совместно циркулируют по всей Северной и Южной Америке, вызывая переплетающиеся синдромы фебрилов, которые ставят под сомнение этиологическую диагностику исключительно на основе клинических критериев. Множественные молекулярные платформы на основе картриджа открывают возможности для децентрализованного тестирования в гиперэпидемионных режимах, однако независимые реальные оценки их клинической и аналитической эффективности остаются ограниченны

Совпадение в аннотации оригинала
Background/Objectives: Arboviruses including dengue virus (DENV), Zika virus (ZIKV), chikungunya virus (CHIKV), yellow fever virus (YFV), and West Nile virus (WNV) co-circulate across the Americas, generating overlapping febrile syndromes that challenge etiological diagnosis based solely on clinical criteria. Cartridge-based multiplex molecular platforms off…

No-known-vector flaviviruses exhibit diverse replication and virulence phenotypes in mice.

UNLABELLED: The Orthoflavivirus genus (flaviviruses) includes globally significant arboviruses, which cycle between arthropod vectors (mosqu … laviviruses that cause human disease are transmitted by mosquitoes and ticks (e.g. West Nile virus, yellow fever virus, tick-borne encephalitis virus). But there are related flaviviruses that are not known to infect arthropods, so are thought to spread by vector-independent routes (no-known-vector flaviviruses, NKVFVs). Not much is known about NKVFVs, but they …

Seroprevalence of Zika virus and dengue virus infections in migrants in Italy.

INTRODUCTION: Estimating the burden of Zika virus (ZIKV) and dengue virus (DENV) infections in migrants is important to promote their health … had nAbs to another orthoflavivirus (n=25/27, 92.59%) such as West Nile virus (WNV) (n=17/25, 68%), Yellow fever virus (YFV) (n=7/25, 28%) and Usutu virus (USUV) (n=1/25, 4%). DISCUSSION: A high prevalence of anti-orthoflavivirus IgG, especially against DENV, was found in the migrant population studied, but no infections were detected. With the recent outbrea …

High prevalence of canine heartworm, Dirofilaria immitis, in pet dogs in south Texas, USA, with evidence of Aedes aegypti mosquitoes contributing to transmission.

BACKGROUND: The canine heartworm Dirofilaria immitis, a filarioid nematode of dogs and other carnivores, is widespread in the USA and the world. Over 20 different mosquito species serve as intermediate hosts of D. immitis, but their contribution to transmission varies according to factors like host feeding patterns, geographic locations and climatic conditions. The yellow fever mosquito, Aedes aegypti, is a competent vector of D. immitis but is often dismissed as a vector of veterinary relevance given its anthropophilic feeding behavior. We evaluated the prevalence of D. immitis in pet dogs al…

Ecology of Aedes vittatus (Diptera: Culicidae) in rock pools across agroecosystem in Northern Savanna, Nigeria.

BACKGROUND & OBJECTIVES: This study focuses on modulating dexterity of some ecological variables of Aedes vittatus classically breeding in r … elbergs will go a long way in decimating population of Ae. vittatus and limit the risk of spread of yellow fever hitherto areas not thriving.

An updated and comprehensive review of the antiviral potential of essential oils and their chemical constituents with special focus on their mechanism of action against various influenza and coronaviruses.

Essential oils and their chemical constituents have been reported with well documented antimicrobial effects against a range of bacterial, f … oil of Lippia species at a concentration of 11.1 μg/mL has been shown to induce 100% inhibition of yellow fever virus in Vero cells. Essential oils and oleoresins have been shown through in vitro and in vivo experiments to induce antiviral effects against Coronavirus infectious bronchitis virus. A study reported 221 phytochemical compounds and essential oils …

Gastric Ulceration and Immune Suppression in Weaned Piglets Associated with Feed-Borne Bacillus cereus and Aspergillus fumigatus.

As a multifactorial cause, gastric ulceration-mediated diarrhea is widely prevalent in the weaned piglets, impairing pig health and economic benefits. With full implementation of antibiotic stewardship programs in China, Bacillus cereus (B. cereus) and Aspergillus fumigatus (A. fumigatus) were identified frequently in porcine feedstuffs and feeds of the animal industry. Association between feed-borne B. cereus and frequent diarrhea remains unclear. In the present study, we conducted a survey of B. cereus and A. fumigatus from feeds and feedstuffs in pig farms during hot season. Interestingly,

RNA Structure Duplication in the Dengue Virus 3' UTR: Redundancy or Host Specificity?

Flaviviruses include a diverse group of medically important viruses that cycle between mosquitoes and humans. During this natural process of … oup of arthropod-transmitted viruses, including important human pathogens such as the dengue, Zika, yellow fever, and West Nile viruses. The natural alternation of these viruses between vertebrate and invertebrate hosts shapes the viral genome population, which leads to selection of different viral variants with potential implications for epidemiological fitne …

Pichia pastoris-Expressed Bivalent Virus-Like Particulate Vaccine Induces Domain III-Focused Bivalent Neutralizing Antibodies without Antibody-Dependent Enhancement in Vivo.

Dengue, a significant public health problem in several countries around the world, is caused by four different serotypes of mosquito-borne dengue viruses (DENV-1, -2, -3, and -4). Antibodies to any one DENV serotype which can protect against homotypic re-infection, do not offer heterotypic cross-protection. In fact, cross-reactive antibodies may augment heterotypic DENV infection through antibody-dependent enhancement (ADE). A recently launched live attenuated vaccine (LAV) for dengue, which consists of a mixture of four chimeric yellow-fever/dengue vaccine viruses, may be linked to the induct

Direct sequencing and expression analysis of a large number of miRNAs in Aedes aegypti and a multi-species survey of novel mosquito miRNAs.

BACKGROUND: MicroRNAs (miRNAs) are a novel class of gene regulators whose biogenesis involves hairpin structures called precursor miRNAs, or … ll-scale cloning efforts. RESULTS: We obtained approximately 130, 000 small RNA sequences from the yellow fever mosquito, Aedes aegypti, by 454 sequencing of samples that were isolated from mixed-age embryos and midguts from sugar-fed and blood-fed females, respectively. We also performed bioinformatics analysis on the Ae. aegypti genome assembly to identify …

Port health problems.

As quarantine control of international travellers dies away, health screening of immigrants becomes more important. Port health controls att … gainst typhoid and infectious hepatitis, when appropriate. Vaccination against smallpox, cholera or yellow fever may be obligatory and a valid certificate required; inoculation against diphtheria, poliomyelitis or tetanus may be sensible, and against rabies if the traveller may come into contact with animals abroad. Although complex queries about health hazard …

Potential Role of Flavivirus NS2B-NS3 Proteases in Viral Pathogenesis and Anti-flavivirus Drug Discovery Employing Animal Cells and Models: A Review.

Flaviviruses are known to cause a variety of diseases in humans in different parts of the world. There are very limited numbers of antivirals to combat flavivirus infection, and therefore new drug targets must be explored. The flavivirus NS2B-NS3 proteases are responsible for the cleavage of the flavivirus polyprotein, which is necessary for productive viral infection and for causing clinical infections; therefore, they are a promising drug target for devising novel drugs against different flaviviruses. This review highlights the structural details of the NS2B-NS3 proteases of different flaviv

Development and validation of a sample-to-result real time multiplex RT-PCR test for human African trypanosomiasis and arboviral febrile illnesses in the Democratic Republic of the Congo.

BACKGROUND: Human African trypanosomiasis (HAT) is a fatal vector-borne disease caused by Trypanosoma brucei. Although HAT incidence has declined, meeting WHO's elimination targets remain difficult, particularly due to limited diagnostic sensitivity for low-parasite load-infections. Arboviruses such as dengue (DENV 1-4), chikungunya (CHIKV), and yellow fever (YFV) virus, present with nonspecific febrile symptoms similar to HAT and are underdiagnosed in Sub-Saharan Africa. Due to this overlap in symptoms and a suspected geographical overlap of vectors and pathogens in the Democratic Republic of…

Borrelia miyamotoi in Saint Petersburg and Leningrad Oblast, Russia: A Multi-Level Assessment of Ticks, Rodents, and Human Exposure.

Background:Borrelia miyamotoi is an emerging tick-borne pathogen causing relapsing fever in humans. Although Saint Petersburg and the surrounding Leningrad Oblast harbor a high abundance of ixodid ticks (I. ricinus, I. persulcatus), no integrated assessment has yet simultaneously addressed pathogen circulation in vectors, reservoir hosts, and human populations in this specific northwestern region of Russia. Methods: During 2022-2024, we collected 1518 questing adult ticks and trapped 516 small mammals in Saint Petersburg and Leningrad Oblast. B. miyamotoi DNA was detected by real-time PCR. Ser

Reduction in Aedes aegypti Population After a Year-Long Application of Targeted Sterile Insect Releases in the West Valley Region of Southern California.

Aedes aegypti is of great public health concern because of its vectorial capacity to transmit various arboviruses such as Zika, yellow fever, dengue, and chikungunya. In California, its expanding geographic distribution has been unrestrained. This urgently calls for innovative tools such as the use of sterile insect technique (SIT) to strengthen invasive Aedes control. This study aimed to determine the efficacy of combining an SIT application into integrated vector management (IVM) strategies in invasive Aedes control in the West Valley region of southern California. A total of 25 Aedes hotspo…

Genotypic and phylogeographic insights into a pre-epidemic variant of Wesselsbron virus detected in sylvatic Aedes mcintoshi from Semuliki Forest, Uganda.

Wesselsbron virus (WSLV) is a neglected mosquito-borne virus within the yellow fever subgroup in the genus Orthoflavivirus of the Flaviviridae family. Despite being primarily a veterinary pathogen able to cause stillbirths, congenital malformations, and mortality in ruminants, WSLV also infects humans, causing a usually self-limiting febrile illness, or may lead to neurological complications in rare cases. WSLV causes sporadic outbreaks in Southern Africa, but findings in mosquitoes from other African countries suggest a wider distribution. Here, we report the detection and isolation of WSLV f…