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Stratum corneum removal facilitates experimental sensitization to mite allergens in atopic dogs.

In humans with atopic dermatitis and in mouse models of IgE-mediated allergic diseases, evidence is mounting that the stratum corneum (SC) provides an important barrier against environmental allergens. At this time, it is not known whether the SC has a similar role in dogs, especially in those with atopic dermatitis. The objectives of this pilot study were to determine whether SC removal led to earlier and stronger sensitization of atopic dogs to Dermatophagoides farinae (Df) house dust mites. Five Maltese-beagle atopic (MBA) dogs were sensitized epicutaneously after the SC was removed with te

A rational clinical approach to suspected insulin allergy: status after five years and 22 cases.

AIMS: Allergy to recombinant human (rDNA) insulin preparations is a rare complication of insulin therapy. However, insulin preparations contain several allergens, and several disorders can resemble insulin allergy. Studies evaluating the diagnostic procedures on suspected insulin allergy are extremely few. METHODS: Since January 1998, we have used a standardized investigative procedure during admittance to the medical ward allowing observation and repeated recording of reactions to intradermal skin test (performed with a commercially available kit containing isolated insulin allergens). Data

Sensitization of naive beagles by intradermal injection of an ascaris antigen: induction of a model of skin allergy.

Ascaris suum-hypersensitive beagles represent a unique model of skin allergy. Despite its suitability, the need to test numerous dogs prior to the selection of natural positive responders causes many complications. We hypothesized that the model could be induced in adult beagles by primary sensitization. Ten dogs were included in an intradermal sensitization program using ascaris antigen and were thereafter tested for cutaneous reaction. After 2 weeks, 60% developed a positive reaction that lasted for months. We therefore present a straightforward method to establish the Ascaris canine model o

An anaphylactoid reaction following local anesthesia: a case report.

A case report of an adverse reaction to a preparation of an amide local anesthetic, prilocaine with epinephrine, is presented. Signs and symptoms were consistent with an anaphylactic reaction and the patient responded positively to treatment based on this assumption. Treatment included administration of epinephrine injected sublingually and oxygen by inhalation. However, subsequent skin testing failed to confirm this diagnosis. A number of explanations are possible and a final diagnosis of an anaphylactoid reaction was made. Local anesthetic allergies and their management are reviewed. The lit

Passive cutaneous anaphylaxis: improvement of quantitative analysis by radioactively labelled antigen.

Experiments were performed to improve the quantitative analysis of passive cutaneous anaphylaxis (PCA) by labelling the antigen with radioactive iodine. After intradermal injection of antibodies and intravenous administration of labelled antigen in rats a close correlation was found between the radioactivity measured in skin biopsies and the amount of antigen as well as the amount of antibodies. This direct dependence makes it possible to improve quantitatively the reading of PCA test results. A quantitative correlation was also found when radioactivity is measured over the skin by scanning pr

Contact and bacterial allergy in children with atopic dermatitis.

Cell-mediated immunity to bacterial antigens was studied by intradermal testing, and to contact antigens (cosmetics, environmental chemicals and topical medicaments) by patch testing in 270 children with atopic dermatitis. The incidence of delayed-type immune cutaneous reactions in these patients was lower than in the controls. Contact allergy is a rare finding in the first 4 years of life but its incidence increases subsequently. In subjects with atopic dermatitis the incidence of sensitization by contact with allergens contained in topical medicaments proved to be lower than in subjects with

Updated EAACI Statement on Drug Hypersensitivity Skin Testing: Methodology and Non-Irritative Concentrations.

These updated EAACI guidelines aim to standardize skin testing methodologies for both immediate and non-immediate drug hypersensitivity reactions. For immediate reactions, the optimal testing window is 4-6 weeks post-reaction; whereas beyond 6 months, false-negative results increase. For chemotherapy-related reactions, earlier testing (within 2 weeks) remains necessary due to treatment urgency. The previously published multicenter ENDA study established the international reference method for positive immediate intradermal test reading: injection of exactly 0.02 mL with a wheal at 20 min measur

Comparative Evaluation of ELISA Tests for Bovine Tuberculosis Detection: Implications for Improved Disease Control.

Accurate diagnosis of bovine tuberculosis (bTB) remains a critical challenge for disease control and eradication programs. While cell-mediated immune (CMI) response tests such as the comparative cervical intradermal tuberculin test (CCITT) are widely used, their sensitivity is limited, especially in later stages of infection. Antibody-based assays, such as enzyme-linked immunosorbent assays (ELISAs), may offer complementary detection and improve case identification. This study aimed to comparatively evaluate two indirect ELISA tests for the detection of antibodies against Mycobacterium bovis i

Diagnostic Accuracy of Skin Test in Patients With Cefaclor-Induced Anaphylaxis.

BACKGROUND: The frequency of cefaclor-induced immediate hypersensitivity reactions has increased in Korea. Skin prick tests (SPTs) and intradermal tests (IDTs) using cephalosporins are not as well validated as those using penicillin. Limited data are available regarding the diagnostic value of SPTs and IDTs for cefaclor-induced immediate hypersensitivity reactions. OBJECTIVE: To compare the clinical utility of the SPT and IDT with that of the ImmunoCAP test in patients with cefaclor-induced immediate hypersensitivity reactions. METHODS: We conducted a retrospective analysis of participants f…

Immediate Articaine Allergy in Pediatric Dentistry: A Case Report of IgE-Mediated Hypersensitivity.

Local anesthetics (LAs) are widely used in dentistry and minor surgical procedures in children and are classified into two groups: esters an … rgological evaluation, guided by a thorough clinical history, involves performing skin prick tests, intradermal tests, and drug provocation tests based on the results of initial skin testing, in accordance with current guidelines. In our patient, the provocation test confirmed an IgE-mediated allergy to the LA, evidenced by cutaneous reactions and bronchospasm, alo …

Assessing cross-reactivity to neuromuscular blocking agents by skin and basophil activation tests in patients with neuromuscular blocking agent anaphylaxis.

BACKGROUND: Following diagnosis of neuromuscular blocking agent (NMBA) anaphylaxis, identifying safe alternatives for subsequent anaesthesia is critical. A patient with anaphylaxis to one NMBA can also have an allergic reaction to other NMBAs (cross-reactivity). Whilst drug provocation testing is standard for identifying or excluding allergy, there is significant risk. In vitro, after an allergen activates basophils, basophils express surface activation markers that can be measured by basophil activation testing (BAT). We compared cross-reactivity between NMBAs assessed by BAT against that by

In vitro T-cell responses to beta-lactam drugs in immediate and nonimmediate allergic reactions.

BACKGROUND: beta-Lactam drugs may induce both cellular and humoral allergic reactions, and there is evidence that T cells play an important role in the pathogenesis of these reactions. The aim of this work was to assess the sensitivity and specificity of the lymphocyte transformation test (LTT) as an in vitro diagnostic tool, in patients with either an immediate or a nonimmediate reaction to penicillin G and/or amoxicillin. METHODS: Fifty patients with a well-documented history of allergic reactions to beta-lactams (31 immediate and 19 nonimmediate) were studied by means of skin tests (prick

Food allergy and idiopathic nephrotic syndrome.

Patients with the idiopathic nephrotic syndrome (INS) with steroid treatment failure, who did or did not have a history suggesting food intolerance, were systematically evaluated for food allergy. We evaluated 42 cases for sensitization to usual foods. The following results were obtained: skin tests (intradermal, Stallergenes Lab) were positive in 16 out of 42 cases; total serum IgE was elevated in 12 of 42 patients; specific IgE (RAST) was positive in eight of 42 patients; the basophil histamine release test (HRT) was positive in 20 of 42 patients. For each of these tests, the results in the

С помощью аллергической реакции на острелизумаб в множестве склероза: ретроспективное когортное исследование.

Окрелизумаб, гуманизированный антиконглональный антитело антиCD20, широко используется в множестве склероза (MS). Реакции, связанные с вливанием (ВКР), широко распространены, в то время как аллергические реакции с помощью аллергии на ИГЭ встречаются редко и плохо характеризуются. ЦЕЛИ: Оценка частотности и факторов риска аллергических реакций на острелизумаб у пациентов с синдромом MS. Мы ретроспективно проанализировали пациентов с синдромом MS, лечившихся окролизумаб (2018 - 2024 годы) в одном центре. Твердые СИР (класс ≥3) прошли аллергологическую оценку, в том числе анализы на кожные кости

Совпадение в аннотации оригинала
BACKGROUND: Ocrelizumab, a humanized anti-CD20 monoclonal antibody, is widely used in multiple sclerosis (MS). Infusion-related reactions (I … ter. Severe IRRs (grade ≥3) underwent allergologic assessment, including skin prick tests (SPT) and intradermal tests (IDT). Predictors of IgE-mediated reactions were explored. RESULTS: Among 387 patients, 94 (24.3%) developed IRRs; 19 (4.9%) were grade 3. Six cases (1.6%) were classified as IgE-mediated based on positive IDT and clinical presentation; all had neg …

Sacroiliac Joint Dysfunction Treated Using Neural Therapy to the Temporomandibular Joint: A Case Report.

Temporomandibular joint disorders (TMJD) include temporomandibular joint dysfunction and bruxism. Sacroiliac joint dysfunction (SJD) is a frequent cause of non-discogenic low back pain. Studies suggest a relationship between TMJD and SJD; however, the link remains unclear. Neural therapy (NT) utilises local anaesthetic injections to treat pain by normalising a dysfunctional autonomic nervous system held responsible for initiating or propagating chronic pain. A 31-year-old female presented with a 1-year history of mechanical left-sided low back pain and sleep bruxism. Examination revealed crepi

Delayed anaphylaxis to red meat masquerading as idiopathic anaphylaxis.

Anaphylaxis is traditionally recognized as a rapidly developing combination of symptoms that often includes hives and hypotension or respira … , pork, lamb, and milk, and the lack of IgE to chicken, turkey, and fish. Skin prick tests (but not intradermal tests) generally are negative. Management of these cases, now common across the southeastern United States, consists of education combined with avoidance of both ingestion of red meat and further tick bites.

A retrospective, multicentric controlled study of the effect of specific allergen immunotherapy on medication needs in cats with atopic skin syndrome.

BACKGROUND: Intradermal allergen testing (IDT) and allergen-specific immunotherapy (ASIT) remain underrated in feline dermatology. HYPOTHESIS/OBJECTIVES: The objectives of this retrospective study were to report the results of IDT and the effects of ASIT on the medication needs in a population of 158 cats diagnosed with feline atopic skin syndrome (FASS). MATERIALS AND METHODS: FASS was diagnosed after the exclusion of other pruritic conditions. IDT was performed under sedation/anaesthesia in 158 cats; in 136 of them, intravenous fluorescein and a Wood's lamp were used to facilitate interpre

Discrepancies between the responses to skin prick test to food and respiratory antigens in two subtypes of patients with irritable bowel syndrome.

AIM: To compare the response to skin prick tests (SPTs) to food antigens (FAs) and inhalant allergens (IAs) in patients with two subtypes of irritable bowel syndrome (IBS) and healthy controls. METHODS: We compared the results of SPTs for IAs and FAs in 87 volunteers divided into three groups: diarrhea predominant IBS (D-IBS) Group I (n = 19), constipation predominant IBS (C-IBS) Group II (n = 17), and normal controls Group III (n = 51). RESULTS: Of the 285 tests (171 for FAs and 114 for IAs) performed in Group I we obtained 45 (26.3%) positive responses for FA and 23 (20.1%) for IA. Of the

[Human seminal plasma allergy type I].

Human seminal plasma allergy (HSPA) is a rare allergic reaction to specific protein fractions of seminal plasma, whereof PSA seems to be a relevant allergen. Predominantly Type I-immunoreactions can occur. The main symptoms are localized and generalized urticaria and sometimes anaphylactic symptoms. The diagnosis is based on history, skin tests and on the determination of specific IgE-levels for (un)fractionated seminal plasma. Here we report a patient with recurrent episodes of generalized urticaria after unprotected sexual intercourse and positive prick-test-reaction on seminal plasma.