Результаты поиска

Молекулярное исследование в Q Fever: обнаружение на основе ПКР ожогов Коксиеллы из ретроспективного исследования клинических образов в больнице третьей инстанции Северной Индии.

Q лихорадка, вызванная Coxiella signetii, является недостаточно диагностированной зоонотической болезнью, имеющей значительные последствия для здоровья населения. Серологические тесты остаются диагностическим краеугольным камнем, но зачастую не дают результатов при ранней инфекции. В этом исследовании изучается полезность цепной реакции полимеразы (ПХР), ориентированной на ген IS1111 для молекулярного обнаружения C. sergetii в клинических образцах человека в условиях ограниченных ресурсов. МЕТОДЫ: Было проведено ретроспективное исследование на основе ПЦР на 243 архивных клинических образцах, с

Совпадение в аннотации оригинала
BACKGROUND: Q fever, caused by Coxiella burnetii, is an underdiagnosed zoonotic disease with significant public health implications. Serolog … f the positive cases belonged to the patients in 1-15 year age group, and two had coinfections with Orientia tsutsugamushi. One case showed coinfection with spotted fever group rickettsia. Clinical features included fever (100%), myalgia (75%), and rash (25%). Phylogenetic analysis revealed that the isolates clustered with the Z3055 reference strain, indic …

Indicators of severe prognosis of scrub typhus: prognostic factors of scrub typhus severity.

BACKGROUND: Scrub typhus is an acute disease, characterized by symptoms of fever, which occurs due to infection by Orientia tsutsugamushi. In most cases, patients recover from the disease with appropriate treatment, but serious and fatal complications may occur. The present study examined laboratory findings and tumor necrosis factor-alpha (TNF-α) levels of scrub typhus patients to identify the prognostic predictors of disease severity. METHOD: Patients whose scrub typhus diagnosis was confirmed by elevated indirect fluorescent antibody (IFA) levels and positive polymerase chain reaction (PCR…

Serosurveillance of scrub typhus in small mammals collected from military training sites near the DMZ, Northern Gyeonggi-do, Korea, and analysis of the relative abundance of chiggers from mammals examined.

Comprehensive quarterly serosurveillance on scrub typhus in small mammals collected from military training sites located near the Demilitari … icus (0.3%, n = 4), and Myodes regulus (0.3%, n = 4) were assayed for the presence of antibodies to Orientia tsutsugamushi. O. tsutsugamushi antibodies were detected in 6 of 8 species and seroprevalence determined; A. agrarius (45.6%), M. musculus (23.1%), M. fortis (48.4%), M. minutus (50.0%), T. triton (50.0%), and R. norvegicus (25.0%). A total of 31,18 …

mNGS helped diagnose scrub typhus presenting as a urinary tract infection with high D-dimer levels: a case report.

BACKGROUND: Scrub typhus is caused by O. tsutsugamushi and spreads through mite larvae biting the skin. Classic symptoms of the disease are … isted and were followed by increased urination duration, frequency, urgency and dysuria for 2 days. Orientia tsutsugamushi was confirmed as the pathogen responsible for the infection through mNGS analysis of her blood samples from Day 13 onwards. The patient's temperature changed remarkably 24 h after the initiation of doxycycline. Over the next 48 h (i.e. …

Scrub Typhus Outbreak in a Remote Primary School, Bhutan, 2014.

Scrub typhus in Bhutan was first reported in 2009. We investigated an outbreak of scrub typhus in a remote primary school during August-October 2014. Delay in recognition and treatment resulted in 2 deaths from meningoencephalitis. Scrub typhus warrants urgent public health interventions in Bhutan.

Optimal Cutoff Titers for Indirect Immunofluorescence Assay for Diagnosis of Scrub Typhus.

We determined the optimal cutoff titers in admission and convalescent-phase samples for scrub typhus indirect immunofluorescence assay using Bayesian latent class models. Cutoff titers of ≥1:3,200 in an admission sample or of a ≥4-fold rise to ≥1:3,200 in a convalescent-phase sample provided the highest accuracy (sensitivity, 81.6%; specificity, 100%).

Multiple organ failure complicating probable scrub typhus.

This report describes a case of life-threatening acute respiratory distress syndrome with multiple organ failure complicating probable scrub typhus. Favorable outcome was associated with fluoroquinolone therapy. Scrub typhus should be suspected in travelers returning from Southeast Asia presenting with unexplained respiratory manifestations.

ELISA-based colorimetric detection of Rickettsia tsutsugamushi DNA from patient sera by nested polymerase chain reaction.

A rapid diagnostic system for scrub typhus was established using colorimetric detection of nested polymerase chain reaction (PCR). This system relied on binding the amplified DNA via a sequence in one of oligodeoxyribonucleotide to the DNA-binding protein GCN4 coated on the well of a micotiter dish. The primer pairs used for the nested PCR were designed on the basis of the homologous nucleotide sequence of the gene that encodes the 56 kDa antigen of serovariants. With this colorimetric PCR, diagnosis can be performed easily from serum samples of patients before the antibody titer increases or

The reactivity between rickettsiae and Weil-Felix test antigens against sera of rickettsial disease patients.

Of the sera which were positive to Rickettsia tsutsugamushi by indirect immunoperoxidase test, approximately 80% sera were positive to a Proteus OXK antigen by Weil-Felix test at 10 or more days after the onset of fever, while only 10% sera were positive within 9 days from the onset of fever. In ELISA using the OXK antigen, almost all of the paired sera of tsutsugamushi disease (TD) patients increased on the IgM antibody titres with the rise of their titres by Weil-Felix test, whereas the IgG antibody titres of these sera were unrelated with the titres of Weil-Felix test. We suspect that the r

Comparative evaluation of the indirect immunoperoxidase test for the serodiagnosis of rickettsial disease.

An indirect immunoperoxidase test was compared with an indirect fluorescent antibody test and the Weil-Felix OXK test for serodiagnosis of scrub typhus by measuring the rickettsial antigen specific activity of IgG, IgM, and whole globulin. Acute and convalescent sera from 50 Rickettsia tsutsugamushi isolate-positive scrub typhus patients and from 45 febrile patients diagnosed as having diseases other than scrub typhus were tested. The receiver operating characteristic for each test showed that the indirect immunoperoxidase and indirect fluorescent antibody tests were more sensitive and specifi

Antigens of virulent and attenuated Rickettsia tsutsugamushi.

We studied the antigens present in L929 mouse fibroblast or rabbit testicular cells, which had been infected or not with a prototype strain of Rickettsia tsutsugamushi, the causal agent of scrub typhus, and its attenuated variant. Immunoblotting revealed four antigens, designated 1, 1a, 2 and 3, which appeared to be specifically associated with infection with this organism. Antigens 1 and 1a had similar mol. wt of about 60 kD and antigen 2 and 3 had mol. wts of 45 kD and 28 kD respectively. Whereas antigen 1a, 2 and 3 were common to infection with either the virulent or the attenuated strains

Serodiagnosis of tsutsugamushi fever (scrub typhus) by the indirect immunoperoxidase technique.

The indirect immunoperoxidase technique was assessed for the serodiagnosis of tsutsugamushi fever (scrub typhus). The antigens were peritoneal smears prepared from mice infected intraperitoneally with the Karp, Kato, and Gilliam strains of Rickettsia tsutsugamushi. Treatment of the mice with cyclophosphamide apparently increased the number of the rickettsiae, and it minimized the exudate that interfered with the specific staining. The rickettsiae were seen as clusters in the juxtanuclear region of the mesothelial cells and also as free particles outside of the cells. By the indirect immunopero

Scrub typhus in the Eastern Solomon Islands and Northern Vanuatu (New Hebrides).

Rickettsia tsutsugmushi has been isolated from Rattus rattus from Vanua Lava island in Northern Vanuatu (New Hebrides) and from R. exulans and Leptotrombidium akamushi on Ndende island in the Eastern Solomon Islands. The well-known vector mite L. deliense was found on Mota Lava and Vanua Lava in Vanuatu, but no isolation was made from pools of this mite. Serology confirms that R. tsutsugamushi infects humans in the Banks group of islands in Northern Vanuatu and that infection is much more widespread in the Solomon Islands than the limited isolations indicate.

Vole agent identified as a strain of the trench fever rickettsia, Rochalimaea quintana.

The vole agent described by Baker in 1946 was studied as an example of a bacterium that has been mistakenly regarded a rickettsia. Unlike rickettsiae, the vole agent killed chicken embryos with great irregularity, multipled primarily at the surface of avian or mammalian cells and not intracellularly, produced colonies rather than plaques on chicken embryo monolayers under agar, and developed small colonies after 4 to 7 days of cultivation on blood plates. It was most conveniently cultivated on monolayers of irradiated L cells and was purified by minor modifications of the Renografin gradient p

Presence of antibodies to scrub typhus and murine typhus in dogs from Selangor, Peninsular, Malaysia.

Dog sera, collected from different communities throughout Selangor, Peninsular Malaysia, were investigated for the presence of antibodies to R. tsutsugamushi and R. typhi. Scrub typhus antibodies were present in animals from the rural areas only, whereas murine typhus antibodies were observed in equal numbers of dogs from both rural and metropolitan areas. Greater percentage of dogs from suburban areas had demonstrable antibody titers to murine typhus than from the urban area.

Cytological investigation of Rickettsia tsutsugamushi infection of mice with different allotypic susceptibility to the agent.

Cytological and cytochemical studies on Swiss and random-bred white mice susceptible to Rickettsia tsutsugamushi infection as well as resistant C57 Black 6 and CBA mice revealed a clear-cut relationship between the severity of infection and the pattern of macrophage reaction. In highly susceptible mice the fatal infection was accompanied by death of macrophages and necrotisation of the peritoneal exudate cells. The resistant mice showed no clinical signs but developed an intensive macrophage reaction, and the main mass of the inoculated rickettsiae died at the inoculation site, the macrophages

Clinical profile and predictors of severity in infantile scrub typhus: A prospective observational study from a tertiary care teaching hospital in Southern India.

BACKGROUND OBJECTIVES: Scrub typhus (ST), a tropical infection caused by Orientia tsutsugamushi , has re-emerged as a global concern in the Asia-Pacific region. Limited data on ST in infants reveals unique presentations, complications, and severity predictors. This study was conducted to evaluate the occurrence of ST in infants, comparing clinical, laboratory and outcomes with ST in older children. METHODS: This is a prospective cohort study conducted in the Pediatrics Department of a teaching hospital in South India. Children <12 years of age presenting with fever for >5 days with serology c…

Ocular Manifestations of Scrub Typhus and Their Association with In-Hospital Mortality.

Scrub typhus, caused by Orientia tsutsugamushi, is a major cause of acute febrile illness in Asia and is associated with significant morbidity and mortality. A prospective observational study (July 2021 to October 2022) was conducted at a tertiary referral center in Northern India to determine the incidence of ocular manifestations and their association with in-hospital mortality. Patients aged ≥12 years with laboratory-confirmed scrub typhus were enrolled. Ocular examinations were performed within 24 hours of admission by a trained emergency physician and independently validated by an ophthal…

Molecular diagnosis and duration of eschar swab sample positivity post-doxycycline therapy in scrub typhus patients.

BACKGROUND: An eschar is not always present in all scrub typhus patients. Furthermore, such patients may present to tertiary care hospitals after administration of doxycycline. The present study aimed to determine the usefulness of using the swab from eschar sites in the diagnosis of scrub typhus in patients who present post-doxycycline therapy. METHODS: Blood, eschar scraping and swab samples were collected daily until patient discharge/death. Real-time SYBR green PCR targeting the groEl gene, TaqMan probe PCR targeting the 47 kDa gene and nested PCR targeting the 56 kDa gene of Orientia tsu…