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Cost-effective treatment of uncomplicated gonorrhoea including co-infection with Chlamydia trachomatis.

We evaluated the cost effectiveness of treating uncomplicated gonorrhoea in a theoretical cohort of 1000 adults (nonpregnant women of reproductive age, or men) with either intramuscular ceftriaxone 125 mg or a single oral dose of cefixime 400 mg, ofloxacin 400 mg or ciprofloxacin 500 mg. We assumed that all patients were also empirically treated for uncomplicated chlamydial infection, with either a single oral dose of azithromycin 1 g, or oral doxycycline 100 mg twice daily for 7 days. Treatment of gonorrhoea with intramuscular ceftriaxone was found to be the most cost-effective alternative. T

Chlamydia trachomatis in patients who used oral contraceptives and had intermenstrual spotting.

OBJECTIVE: To identify the incidence of positive assays for direct antigen detection of Chlamydia trachomatis in oral contraceptive (OC) users with and without intermenstrual spotting and in women seeking contraception. METHODS: We studied 65 women who had used OCs for more than 3 months and who presented with intermenstrual spotting for which no readily demonstrable cause could be identified. They were compared with 65 matched controls who were taking OCs without intermenstrual spotting and who had chlamydia testing because of one or more risk factors, and 65 matched controls seeking contrac…

Characteristics of gram-stained cervical smear from patients with Chlamydia trachomatis infection.

Cervical discharges from 142 women attending the Public Gynecologic Service of Araraqura (SESA), Brazil were cultured for Chlamydia trachomatis. Gram-smears and plating on semiquantitative sheep blood agar and chocolate agar were also carried out. An isolation rate of 18% was reported. The presence of purulent cervical secretion was observed in 8 (32%) out of 25 women. It was also observed that a substantial proportion of culture-positive women had no symptoms. Our data demonstrate that screening tests should be based on specific diagnostic techniques for Chlamydia trachomatis since the majori…

Management of Chlamydia trachomatis genital infections: reported practices of primary care physicians.

OBJECTIVE: To determine the knowledge of primary care physicians about Chlamydia trachomatis genital infection and its management. DESIGN: Self-administered questionnaire comprising direct questions and hypothetical cases. PARTICIPANTS: All 108 general and family practitioners on the north shore of Vancouver were sent the questionnaire; 79 (73%) responded. RESULTS: There was a reasonable level of knowledge in many areas, particularly among the physicians who had graduated more recently than the others. Virtually all stated that they have access to chlamydial diagnostic testing, and most ind…

Adverse foetal outcome in specific IgM positive Chlamydia trachomatis infection in pregnancy.

A study was undertaken to determine the adverse foetal outcome in antichlamydial IgM positive asymptomatic pregnant females. An indirect immunoperoxidase assay was done to detect IgM in 78 apparently normal asymptomatic pregnant women during the third trimester and follow up was done till delivery to study the effect of chlamydial infection on foetal outcome. A total of 28 (35.9%) women were positive for antichlamydial IgM while only 3.33 per cent asymptomatic non-pregnant normal women (controls) were positive. 28.7 per cent IgM positive mothers delivered low birth weight (LBW) babies (P less

Protective immunity in pig-tailed macaques after cervical infection with Chlamydia trachomatis.

Seven pig-tailed macaques were inoculated in the cervix with Chlamydia trachomatis. Cervical infection resulted in all animals. After spontaneous cessation of cervical shedding of C. trachomatis, repeated inoculation either failed to produce infection, or resulted in infection of shorter duration with lower inclusion counts. Thus, cervical infection may lead to protective local immunity in pig-tailed macaques.

Isolation of Chlamydia trachomatis from prostatic tissue of patients undergoing transurethral prostatectomy.

Chlamydia trachomatis was isolated from three of 100 prostatic tissue specimens obtained from patients undergoing transurethral resection of the prostate. All examined patients were free from symptoms of urethral or prostatic inflammation. Our findings are in accordance with the high incidence of asymptomatic chlamydial infection of the lower genital tract and the increasing evidence that C. trachomatis can cause infection of the prostate.

Differential human serologic response to two 60,000 molecular weight Chlamydia trachomatis antigens.

Chlamydia trachomatis causes sexually transmitted diseases and is associated with serious long-term sequelae such as tubal infertility and ectopic pregnancy. There have been suggestions that chlamydial antigens of approximately 57,000-60,000 Mr may be involved in the immunopathology. Two important chlamydial antigens of 57,000-60,000 Mr are a Triton X-100-soluble antigen, which induces hypersensitivity in ocular models, and a sarcosyl-insoluble cysteine-rich structural protein, omp2. In this study, a 57,000 Mr Triton X-100-soluble protein was characterized as the chlamydial homolog of groEL, a…

Diagnosis of Neisseria gonorrhoeae and Chlamydia trachomatis infections using antigen detection methods.

Rapid antigen detection methods have great potential value in managing sexually transmitted gonococcal and chlamydial infections. Ideally, s … ual men, and 3) in situations requiring lengthy specimen transport. Because culture confirmation of Chlamydia trachomatis infections is not widely available, antigen detection tests could be of great value in management of these infections. Major uses include 1) confirming infection in women with cervicitis, endometritis, and pelvic inflammatory disease; 2) screening f …

Detection of IgM antibodies against Chlamydia trachomatis by enzyme linked fluorescence immunoassay.

A simple, sensitive enzyme linked fluorescence immunoassay has been developed to detect IgM antibodies against Chlamydia trachomatis. Reticulate bodies and elementary bodies from C trachomatis L2/434 Bu strain were isolated and used as antigens in the assay. Of 113 serum samples obtained from infants with pneumonia, 27 (23.9%) had IgM antibodies to C trachomatis L2 reticulate bodies and nine (8.0%) had IgM antibodies to C trachomatis L2 elementary bodies (titre greater than or equal to 1/500). Specific IgM antibodies were not detected in 20 control serum samples obtained from healthy adults an…

Chlamydia trachomatis species-specific epitope detected on mouse biovar outer membrane protein.

A common antigenic determinant on the chlamydial major outer membrane protein was detected on each of the three Chlamydia trachomatis biovars (trachoma, lymphogranuloma venereum, and mouse). This determinant was prominently displayed on the surface of chlamydial strains from both the trachoma and lymphogranuloma venereum biovars. However, detection of this determinant on a mouse biovar strain required denaturation by sodium dodecyl sulfate or periodate oxidation. This determinant provides a definable taxonomic link between the three biovars of C. trachomatis.

Cultures of Chlamydia trachomatis in mouse peritoneal macrophages: factors affecting organism growth.

Growth of Chlamydia trachomatis B/TW-5/OT and L(2)/434/Bu strains in cultures of thioglycolate-activated mouse peritoneal macrophages was studied. Both strains grew to a limited extent in the macrophages, but lymphogranuloma venereum (LGV) grew better than trachoma. Growth was enhanced by centrifugation of the inoculum onto the macrophage cell layer and inhibited by pretreatment of macrophages for 2 h with 100 mug of concanavalin A per ml. No significant effect was observed by pretreatment of macrophages with diethylaminoethyl-dextran (30 mug/ml, 30 min), cytochalasin B (20 mug/ml, 1 h), and c…

[Evaluation of incidence of Chlamydia trachomatis infections among the group of infertile women diagnosed by laparoscopy, and based on properties of Chlamydia trachomatis in the cervical canal, peritoneal fluid and ovarian cyst puncture].

The number of 75 patients who underwent diagnostic et operative laparoscopy was analysed. The percentage of infection cases caused by Chlamydia Trachomatis was 9.33% investigations proved correlation between fallopian tube factor and infection. It seems that pelvic infection not always undergoes with infection of uterine cervix.

Chlamydia trachomatis-specific interferon-γ-producing CD8 T-cells are associated with lower chlamydia bacterial load in reinfected women.

This study aimed to better understand the importance of CD8 T cell responses in protective immunity to chlamydia. In women evaluated for reinfection at a 3-month follow-up visit after treatment for chlamydia, the presence or magnitude of Chlamydia trachomatis-specific CD8 interferon-gamma (IFN-γ) responses to Momp and Pgp3 peptide pools was not associated with reinfection status, despite having an increased frequency of responses compared to C. trachomatis CD4-specific T cells. However, reinfected women with detectable interferon-gamma (IFN-γ)-producing CD8 T cells had lower C. trachomatis bac…

M13 phages engineered with chlamydia phage φCPG1 protein IN5 and arginine-glycine-aspartic acid inhibits Chlamydia trachomatis intracellular growth.

Chlamydia trachomatis (C. t) is the most common causative agent of sexually transmitted bacterial urogenital infections worldwide. C. t treatment failure is increasing because antibiotic resistance has developed in recent years. Therefore, the development of novel therapeutic strategies is necessary. Here, we constructed an M13 phage carrying two functional peptides, including the integrin binding peptide arginine-glycine-aspartic acid (RGD) on pⅧ and the IN5 protein from Chlamydia caviae phage φCPG1 on pIII to reduce C. t infection. We called these phages M13-RGD8-IN53. The recombinant phages…

Does population screening for Chlamydia trachomatis raise anxiety among those tested? Findings from a population based chlamydia screening study.

BACKGROUND: The advent of urine testing for Chlamydia trachomatis has raised the possibility of large-scale screening for this sexually transmitted infection, which is now the most common in the United Kingdom. The purpose of this study was to investigate the effect of an invitation to be screened for chlamydia and of receiving a negative result on levels of anxiety, depression and self-esteem. METHODS: 19,773 men and women aged 16 to 39 years, selected at random from 27 general practices in two large city areas (Bristol and Birmingham) were invited by post to send home-collected urine sample…

Последовательность хламидийных трахоматисов с высоким разрешением: улучшенные результаты по клиническим образцам с низким содержанием ДНК Тхоматиса.

Для хламидийных трахоматисов было разработано несколько программ машинописи мультилокационной последовательности (MLST). Схема MLST Bom основана на амплификации PCR и последовательности пяти гипервариантных генов и OmpA. В отличие от других схем хламидии MLST, программа Bom MLST дает более высокое разрешение и филогенетические деревья, которые сопоставимы с деревьями, полученными в ходе целой последовательности генома. Тем не менее, с помощью схемы MLST Бома были получены плохие результаты в клинических пробах с низкой концентрацией ДНК хламидии.Результаты: В этой работе мы представляем усовер

Совпадение в аннотации оригинала
BACKGROUND: Several Multilocus Sequence Typing (MLST) schemes have been developed for Chlamydia trachomatis. Bom's MLST scheme for MLST is based on nested PCR amplification and sequencing of five hypervariable genes and ompA. In contrast to other Chlamydia MLST schemes, Bom's MLST scheme gives higher resolution and phylogenetic trees that are comparable to t…

The Netherlands Chlamydia cohort study (NECCST) protocol to assess the risk of late complications following Chlamydia trachomatis infection in women.

BACKGROUND: Chlamydia trachomatis (CT), the most common bacterial sexually transmitted infection (STI) among young women, can result in serious sequelae. Although the course of infection is often asymptomatic, CT may cause pelvic inflammatory disease (PID), leading to severe complications, such as prolonged time to pregnancy, ectopic pregnancy, and tubal factor subfertility. The risk of and risk factors for complications following CT-infection have not been assessed in a long-term prospective cohort study, the preferred design to define infections and complications adequately. METHODS: In the…