Nature communications · 2023
Intestinal Atp8b1 dysfunction causes hepatic choline deficiency and steatohepatitis.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Tamura Ryutaro, Sabu Yusuke, Mizuno Tadahaya, Mizuno Seiya, Nakano Satoshi, Suzuki Mitsuyoshi, Abukawa Daiki, Kaji Shunsaku, Azuma Yoshihiro, Inui Ayano, Okamoto Tatsuya, Shimizu Seiichi, Fukuda Akinari, Sakamoto Seisuke, Kasahara Mureo, Takahashi Satoru, Kusuhara Hiroyuki, Zen Yoh, Ando Tomohiro, Hayashi Hisamitsu
Аннотация
Choline is an essential nutrient, and its deficiency causes steatohepatitis. Dietary phosphatidylcholine (PC) is digested into lysoPC (LPC), glycerophosphocholine, and choline in the intestinal lumen and is the primary source of systemic choline. However, the major PC metabolites absorbed in the intestinal tract remain unidentified. ATP8B1 is a P4-ATPase phospholipid flippase expressed in the apical membrane of the epithelium. Here, we use intestinal epithelial cell (IEC)-specific Atp8b1-knockout (Atp8b1IEC-KO) mice. These mice progress to steatohepatitis by 4 weeks. Metabolomic analysis and cell-based assays show that loss of Atp8b1 in IEC causes LPC malabsorption and thereby hepatic choline deficiency. Feeding choline-supplemented diets to lactating mice achieves complete recovery from steatohepatitis in Atp8b1IEC-KO mice. Analysis of samples from pediatric patients with ATP8B1 deficiency suggests its translational potential. This study indicates that Atp8b1 regulates hepatic choline levels through intestinal LPC absorption, encouraging the evaluation of choline supplementation therapy for steatohepatitis caused by ATP8B1 dysfunction.