Enhancing 3D food printing precision: Development and interaction behavior of soy protein isolate-konjac glucomannan-xanthan gum composite ink based on hot-melt extrusion.

In the field of 3D printing, the physicochemical properties of composite inks are pivotal for constructing accurate printing networks. However, the precise fabrication of molded simulants using food 3D printing technology remains a challenging endeavor. The molecular structure and rheological properties of soybean protein isolate (SPI) make it prone to fracture under high shear stress, compromising printing accuracy and stability. This study aimed to address the deficiencies in precision and stability of existing soy protein-based inks for plant-based meats by developing a food composite ink system that incorporated SPI, konjac glucomannan (KGM), and xanthan gum (XG). The new ink system was designed to capitalize on the interactions between proteins and polysaccharides, as well as the synergistic effects of polysaccharides, to achieve high printing precision and the potential for simulant preparation. The addition of KGM and XG to the ink formulation enhanced the shear-thinning behavior, which was more amenable to the printing process, compared to the control group consisting of SPI alone. The SK1X1 composite ink demonstrated a significant improvement in printing precision by 40 % and in printing stability by 59 %, with final values reaching 99.11 % and 98.51 %, respectively. Additionally, hydrogen bonding was identified as a predominant factor in the gel network structure of SPI-KGM-XG composite inks. The self-assembling behavior of KGM-XG with SPI resulted in a robust spatial network structure, which in turn enhanced the thermal stability of the ink. In conclusion, the SPI-KGM-XG blends were determined to be suitable for use as thermo-extruded edible inks, and the synergistic effect of KGM-XG bolstered the gel properties of the hybrid inks, positioning them as ideal candidates for application in the 3D printing of meat simulants.