[Listeria pathogenicity factors and their role in pathogenesis and laboratory diagnosis of listeriosis].

The mechanisms of relationships between Listeria and eukaryotic cells on the level of the main biomolecules are analyzed. These mechanisms determine the penetration and multiplication of Listeria and their importance for the pathogenesis of listeriosis. The theoretical and practical aspects of the regulation of the expression of pathogenicity factors in Listeria, connected with the activation of the regulatory protein PrfA and autoregulatory mechanisms, are considered. The elimination of the autorepressor from the cultivation medium results in the activation of the regulatory protein and the induction of the pathogenicity factors. The effective identification method for L. monocytogenes, permitting the differentiation of pathogenic and non-pathogenic Listeria, has been developed on the basis of the study of lecithinase expression in the presence of activated charcoal.