Cell reports · 2021
Diversity and function of motile ciliated cell types within ependymal lineages of the zebrafish brain.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
D'Gama Percival P, Qiu Tao, Cosacak Mehmet Ilyas, Rayamajhi Dheeraj, Konac Ahsen, Hansen Jan Niklas, Ringers Christa, Acuña-Hinrichsen Francisca, Hui Subhra P, Olstad Emilie W, Chong Yan Ling, Lim Charlton Kang An, Gupta Astha, Ng Chee Peng, Nilges Benedikt S, Kashikar Nachiket D, Wachten Dagmar, Liebl David, Kikuchi Kazu, Kizil Caghan, Yaksi Emre, Roy Sudipto, Jurisch-Yaksi Nathalie
Аннотация
Motile cilia defects impair cerebrospinal fluid (CSF) flow and can cause brain and spine disorders. The development of ciliated cells, their impact on CSF flow, and their function in brain and axial morphogenesis are not fully understood. We have characterized motile ciliated cells within the zebrafish brain ventricles. We show that the ventricles undergo restructuring through development, involving a transition from mono- to multiciliated cells (MCCs) driven by gmnc. MCCs co-exist with monociliated cells and generate directional flow patterns. These ciliated cells have different developmental origins and are genetically heterogenous with respect to expression of the Foxj1 family of ciliary master regulators. Finally, we show that cilia loss from the tela choroida and choroid plexus or global perturbation of multiciliation does not affect overall brain or spine morphogenesis but results in enlarged ventricles. Our findings establish that motile ciliated cells are generated by complementary and sequential transcriptional programs to support ventricular development.