The Journal of pharmacy and pharmacology · 2026
The flavonoids of Trichosanthis Fructus in improving ALI mice based on AUF-MS-SPR.
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Xu Cuicui, Yan Qingyu, Kong Hao, Qi Hong, Shi Pengliang, Fu Yanan, Wan Jiafu, Zhao Qitao, Guo Qingmei
Аннотация
OBJECTIVES: To investigate the anti-inflammatory mechanism and potential bioactive compounds of the flavonoids of Trichosanthis Fructus (TF) in LPS-induced acute lung injury (ALI) mice.
METHODS: The active fraction of TF was screened based on nitric oxide (NO) levels in RAW264.7 cells, and its antioxidant capacity was evaluated in BEAS-2B cells. Balb/c mice were intratracheally instilled with LPS (3 mg/kg), and the levels of Interleukin-1β (IL-1β), IL-6, and Tumor necrosis factor alpha (TNF-α) in serum and lung were measured. The targets of TF intervention in ALI were predicted using Western blotting. The compounds that bind specifically to the targets were identified by affinity ultrafiltration mass spectrometry, and then validated the binding specificity using surface plasmon resonance technology.
KEY FINDINGS: The NO level in LPS-induced RAW264.7 was significantly reduced by the ethyl acetate fraction of TF (TF-EA), and the reactive oxygen species level in H2O2-induced BEAS-2B cells was also decreased. Subsequently, TF-EA may significantly improve LPS-induced ALI in mice by EGFR and TLR4-mediated PI3K/Akt signaling pathway. The flavonoids especially rutin, luteolin-7-O-glucoside, apigenin-7-O-glucoside, and diosmetin-7-O-glucoside had better binding with Epidermal growth factor receptor (EGFR) and Toll-like receptor 4 (TLR4).
CONCLUSIONS: The TF-EA may reduce the release of inflammatory factors by affecting the PI3K/Akt signaling pathway mediated by EGFR and TLR4.
METHODS: The active fraction of TF was screened based on nitric oxide (NO) levels in RAW264.7 cells, and its antioxidant capacity was evaluated in BEAS-2B cells. Balb/c mice were intratracheally instilled with LPS (3 mg/kg), and the levels of Interleukin-1β (IL-1β), IL-6, and Tumor necrosis factor alpha (TNF-α) in serum and lung were measured. The targets of TF intervention in ALI were predicted using Western blotting. The compounds that bind specifically to the targets were identified by affinity ultrafiltration mass spectrometry, and then validated the binding specificity using surface plasmon resonance technology.
KEY FINDINGS: The NO level in LPS-induced RAW264.7 was significantly reduced by the ethyl acetate fraction of TF (TF-EA), and the reactive oxygen species level in H2O2-induced BEAS-2B cells was also decreased. Subsequently, TF-EA may significantly improve LPS-induced ALI in mice by EGFR and TLR4-mediated PI3K/Akt signaling pathway. The flavonoids especially rutin, luteolin-7-O-glucoside, apigenin-7-O-glucoside, and diosmetin-7-O-glucoside had better binding with Epidermal growth factor receptor (EGFR) and Toll-like receptor 4 (TLR4).
CONCLUSIONS: The TF-EA may reduce the release of inflammatory factors by affecting the PI3K/Akt signaling pathway mediated by EGFR and TLR4.
Предметные рубрики MeSH
AnimalsFlavonoidsMiceMice, Inbred BALB CLipopolysaccharidesRAW 264.7 CellsAcute Lung InjuryAnti-Inflammatory AgentsNitric OxideToll-Like Receptor 4Signal TransductionMaleSurface Plasmon ResonanceAntioxidantsDisease Models, AnimalErbB ReceptorsFruitTrichosanthesReactive Oxygen SpeciesMass SpectrometryPhosphatidylinositol 3-KinasesLungProto-Oncogene Proteins c-akt