Zhonghua yu fang yi xue za zhi [Chinese journal of preventive medicine] · 2011
[Study of three ciguatera fish poisoning cases in Xiamen city, in 2005].
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Luo He-dong, Bai Yan-yan, Zhou Na
Аннотация
OBJECTIVE: To find out the reason of three ciguatera fish poisoning cases in Xiamen in 2005 and identify the fish species.
METHODS: The grouper implicated in food poisoning and seven other coral reef fishes collected from market were tested by mice bioassay and ciguatoxin-test kit. The mtDNA was extracted from toxic grouper meat, and Cty b gene segment was amplified and the PCR products were sequenced. The sequences were compared with those in the GenBank.
RESULTS: The result turned out to be positive by the ciguatoxin-test kit, while the toxicity of the toxic grouper implicated in food poisoning was 0.11 mouse unit (MU)/g by mice bioassay. A 475 bp segments of Cty b gene was amplified by PCR and the sequence was 99% homologous with Epinephelus fuscoguttatus (GenBank: AY950695).No ciguatoxin in six grouper species collected from market was detected.
CONCLUSION: All three food poisoning cases were caused by consumption of ciguatoxin-carrying groupers.
METHODS: The grouper implicated in food poisoning and seven other coral reef fishes collected from market were tested by mice bioassay and ciguatoxin-test kit. The mtDNA was extracted from toxic grouper meat, and Cty b gene segment was amplified and the PCR products were sequenced. The sequences were compared with those in the GenBank.
RESULTS: The result turned out to be positive by the ciguatoxin-test kit, while the toxicity of the toxic grouper implicated in food poisoning was 0.11 mouse unit (MU)/g by mice bioassay. A 475 bp segments of Cty b gene was amplified by PCR and the sequence was 99% homologous with Epinephelus fuscoguttatus (GenBank: AY950695).No ciguatoxin in six grouper species collected from market was detected.
CONCLUSION: All three food poisoning cases were caused by consumption of ciguatoxin-carrying groupers.