Immune-enhancing effects of enzymatic hydrolysates of peanut sprouts in RAW 264.7 macrophages and cyclophosphamide-induced immunosuppressed mouse model.

Immune deficiency is associated with the development of various diseases. Resveratrol, the main bioactive component of peanut sprouts, exerts immunomodulatory effects. Enzymatic hydrolysis increases the yield of bioactive components from plant resources. In this study, the immune-enhancing effects of three types of peanut sprout extracts (peanut sprout non-enzyme extract (PSNE), peanut sprout cellulase extract (PSCE), and peanut sprout pectinase extract (PSPE)) were evaluated to confirm the effectiveness of enzymatic hydrolysis extract of peanut sprouts. The immune-boosting potency of the extracts was assessed by measuring proinflammatory mediators (nitric oxide (NO), and prostaglandin E2 (PGE2)), inflammatory cytokines (tumor necrosis factor-α (TNF-α), interleukin (IL)-6, IL-1β, and monocyte chemoattractant protein-1 (MCP-1)) in RAW 264.7 cells. To evaluate the immune-boosting efficacy of the extract in an in vivo model, immune organ indices and total leukocyte and natural killer (NK) cell populations were measured in a cyclophosphamide-induced immunosuppressed mouse model. PSCE had a significantly higher resveratrol content than PSNE and PSPE. Moreover, PSCE actively increased NO and PGE2 production in RAW 264.7 cells in a concentration-dependent manner, indicating its immune-promoting potential. PSCE significantly increased the expression of inflammatory cytokines and promoted the phosphorylation and nuclear translocation of nuclear factor kappa-light-chain-enhancer of activated B cells, thereby enhancing immunity. In the mouse model, oral administration of PSCE enhanced immunity by suppressing the cyclophosphamide-induced loss of immune organ index and decline of leukocyte population in the blood and NK cell population in the spleen. Our results suggest that hydrolysis using cellulase can promote the immune-enhancing effects of peanut sprout extract by increasing the extraction of resveratrol.