Molecular immunology · 2024
Baicalin-Geniposide glycosides inhibit PM2.5-induced brain damage in rats via the 5-LOX/LTB4 pathway.
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Zhang Jiahao, Zhao Lu, Zhao Andong, Hu Tian, Zhou Xuewei, Li Yuan, Gong Jie, Wang Chuan, Liu Jiping, Wang Bin
Аннотация
BACKGROUND: PM2.5-induced brain damage is related to systemic inflammation and oxidative stress, which can be caused by PM2.5 acting directly on the brain or indirectly by stimulating inflammation in the peripheral nervous system; however, the underlying pathological mechanisms are still unclear. Baicalin (BC) and geniposide glycosides (GD) are natural products that may exert neuroprotective effects by reducing inflammatory responses and oxidative damage.
METHODS: A mouse model of microglial polarization was established via in vitro exposure to PM2.5, and tracheal drip injection of PM2.5-suspended dust was used to simulate PM2.5-induced brain damage. The expression of polarization markers in mouse microglia, changes in the levels of inflammatory factors, and changes in memory, cerebral blood flow and 5-lipoxygenase/leukotriene B4 (5-LOX/LTB4) pathway proteins in a brain injury model rat were investigated via enzyme-linked immunosorbent assays, real-time fluorescence quantitative PCR and Western blotting techniques after baicalein-gardenia glycoside action.
RESULTS: The most successful experimental animal model of PM2.5-induced brain damage in rats was achieved when the dye dose was 15 mg/kg and the dyeing time was 3 M. BC/GD is protective against PM2.5-induced brain damage in rats. Baicalin and gardenia ameliorate microglial activation and brain tissue inflammatory injury induced by PM2.5, and its protective effect is associated with the 5-LOX/LTB4 pathway.
CONCLUSIONS: Baicalin-Geniposide glycoside prevents PM2.5-induced brain injury by activating the 5-LOX/LTB4 pathway and inhibiting inflammation and microglial polarization to the M2 type.
METHODS: A mouse model of microglial polarization was established via in vitro exposure to PM2.5, and tracheal drip injection of PM2.5-suspended dust was used to simulate PM2.5-induced brain damage. The expression of polarization markers in mouse microglia, changes in the levels of inflammatory factors, and changes in memory, cerebral blood flow and 5-lipoxygenase/leukotriene B4 (5-LOX/LTB4) pathway proteins in a brain injury model rat were investigated via enzyme-linked immunosorbent assays, real-time fluorescence quantitative PCR and Western blotting techniques after baicalein-gardenia glycoside action.
RESULTS: The most successful experimental animal model of PM2.5-induced brain damage in rats was achieved when the dye dose was 15 mg/kg and the dyeing time was 3 M. BC/GD is protective against PM2.5-induced brain damage in rats. Baicalin and gardenia ameliorate microglial activation and brain tissue inflammatory injury induced by PM2.5, and its protective effect is associated with the 5-LOX/LTB4 pathway.
CONCLUSIONS: Baicalin-Geniposide glycoside prevents PM2.5-induced brain injury by activating the 5-LOX/LTB4 pathway and inhibiting inflammation and microglial polarization to the M2 type.