Decoration of Fe3O4-vitamin C nanoparticles on alginate-chitosan nanocomplex: Characterization, safety, bioacessibility boost and Iron Nanofortification in A2 goat milk gels.

In this study, an alginate-chitosan (AL-CS) nanocomplex decorated with vitamin C coated iron oxide nanoparticles (Fe3O4-vit C NPs) was investigated as a novel nanoiron fortification agent. The Fe3O4-vit C NPs decorated on AL-CS nanocomplex underwent comprehensive characterization, including zeta potential, fourier transform infrared spectroscopy, X-ray diffraction, and UV-vis spectroscopy. The transmission electron microscopy (TEM) analysis confirmed the decoration of Fe3O4-vit C NPs on AL-CS nanocomplex. The dynamic light scattering and thermogravimetric analysis showed enhanced thermal properties of decorated nanocomplex than the undecorated control. Biocompatibility testing on HepG2 cell lines revealed improved compatibility, while intestinal Caco2 cell lines showed approximately 51 % greater bioacessibility than controls. Further, 8 mg of Fe3O4-vit C NPs decorated AL-CS nanocomplex nanofortified 80 g of A2 goat milk gels (GMGs) which provided 0.072 mg/g of nanoiron without showing significant changes in texture and color compared to the control A2 GMGs. The PCA analysis helped to identify the impact of various factors for the preparation of decorated nanocomplex.