Hyaluronan as a mediator for the hepatoprotective effect of Diosmin/Hesperidin complex.

Daflon is a phlebotonic drug widely used in chronic venous or lymphatic insufficiency. This study designed to investigate the relation of daflon with hyaluronan as a mediator for the hepatoprotective effect against Carbon tetrachloride (CCl4) and/or γ-radiation induced liver damage. Animals of this study were administered CCl4 (1 ml/kg b.wt.), exposed to γ-radiation (1Gy) and treated with daflon (100 mg/kg/day). Our results showed the ameliorative effect of daflon on cytochrome P450 (CYT P450), lipid peroxidation (MDA), liver enzymes (aspartate amino transferase; AST, alanine aminotransferase; ALT and gamma glutamyl transferase; γ-GT), antioxidant capacity (reduced glutathione; GSH and glutathione per oxidase; GPx), inflammatory markers (C-reactive protein; CRP and interlukin- 6; IL-6), alpha-fetoprotein (AFP) and extra cellular matrix proteins (hyaluronan; HA and hyaluronidase; HAase) which was supported by histopathological examination of liver sections compared to the damage induced in CCL4 and/or rats exposed to radiation. It could be concluded that the hepatoprotective effect of daflon is mediated via antioxidant and anti-inflammatory activity in addition to preserving native tissue hyaluronan by preventing its degradation.