Copper induces cytotoxicity in freshwater bivalve Anodonta woodiana hemocytes.

Hemocytes of freshwater bivalves are an important target model for evaluating copper (Cu) toxicity in vitro, with excess Cu causing adverse responses in these organisms. Despite this, the mechanisms underlying cytotoxicity remain poorly understood. The freshwater bivalve Anodonta woodiana, employed as a model organism in freshwater environments, was utilized in this study. Hemocytes of A. woodiana were exposed to various aqueous Cu treatments (0.001, 0.01, 0.1, 1, and 10 mg/L), and a control group (no Cu added) for 3 h to investigate the cytotoxic mechanisms of Cu. The results showed a significant increase in the production of reactive oxygen species in hemocytes of all Cu exposed groups compared to the control (p < 0.05). Remarkably, Cu treatments disrupted the cellular membrane (p < 0.05) but did not induce significant changes in the stability of the lysosomal membrane. Cu targeted the mitochondria, leading to a reduction in mitochondrial membrane potential. Additionally, all Cu treatments significantly increased the degree of DNA damage (p < 0.05). Cellular damage and a significant decline in cell viability were observed when the Cu exposure concentration reached 0.1, 1, and 10 mg/L (p < 0.05). Our study provides new insights into the cytotoxicity mechanisms triggered by Cu in hemocytes of the freshwater bivalve A. woodiana, even under environmentally relevant conditions of 0.01 mg/L exposure.