H2O2-dependent decolorization of Poly R-481 by particulate fractions from Phanerochaete chrysosporium.

A cell-free preparation from Phanerochaete chrysosporium culture medium decolorized the polymeric dye Poly R-481. The majority of this decolorization activity sedimented when centrifuged at 150,000 X g, indicating that it was associated with a particulate body. The activity was sensitive to heat, azide and cyanide, was stimulated by exogenously added H2O2, and was optimal around pH 4. Electron micrographs of the sedimented culture medium fraction showed the presence of numerous particulate structures. A similar dye decolorization activity from sonicated mycelium also sedimented at 150,000 X g.