International journal of nanomedicine · 2012
Targeted therapy for glioma using cyclic RGD-entrapped polyionic complex nanomicelles.
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Liu Xiaoying, Cui Wenguo, Li Bo, Hong Zhen
Аннотация
BACKGROUND: The purpose of this study was to test the efficacy of cyclic Arg-Gly-Asp (RGD) peptide conjugated with polyionic complex nanomicelles as targeted therapy for glioma.
METHODS: A stable cyclic RGD polyionic complex nanostructure, ie, a c(RGDfC) polyionic complex micelle, was synthesized and its biocompatibility with cultured neurons was assessed using a cell viability assay. Targeted binding to cultured glioma cells was evaluated by the CdTe quantum dot marking technique and a cell viability assay. The inhibitory effect of the nanomicelles against glioma cells was also evaluated, and their targeted migration into rat brain glioma cells and apoptotic effects were traced by the CdTe quantum dot marking and immunohistochemical staining.
RESULTS: c(RGDfC) polyionic complex micelles did not affect the growth of neurons but bonded selectively to and inhibited proliferation of glioma cells in vitro. When tested in vivo, the micelles migrated into glioma cells, inducing apoptosis in the rat brain.
CONCLUSION: The c(RGDfC) polyionic complex micelle is an effective targeted therapy against glioma.
METHODS: A stable cyclic RGD polyionic complex nanostructure, ie, a c(RGDfC) polyionic complex micelle, was synthesized and its biocompatibility with cultured neurons was assessed using a cell viability assay. Targeted binding to cultured glioma cells was evaluated by the CdTe quantum dot marking technique and a cell viability assay. The inhibitory effect of the nanomicelles against glioma cells was also evaluated, and their targeted migration into rat brain glioma cells and apoptotic effects were traced by the CdTe quantum dot marking and immunohistochemical staining.
RESULTS: c(RGDfC) polyionic complex micelles did not affect the growth of neurons but bonded selectively to and inhibited proliferation of glioma cells in vitro. When tested in vivo, the micelles migrated into glioma cells, inducing apoptosis in the rat brain.
CONCLUSION: The c(RGDfC) polyionic complex micelle is an effective targeted therapy against glioma.
Предметные рубрики MeSH
Analysis of VarianceAnimalsAntineoplastic AgentsApoptosisBrain NeoplasmsCadmium CompoundsCell ProliferationCell SurvivalCells, CulturedDose-Response Relationship, DrugDrug Delivery SystemsGliomaHydrogen-Ion ConcentrationImmunohistochemistryMaterials TestingMicellesNeuronsOligopeptidesQuantum DotsRatsRats, WistarTelluriumXenograft Model Antitumor Assays