An ultrasensitive electrochemical sensor for the simultaneous detection of baicalin and baicalein in pharmaceuticals and serum samples.

Developing an accurate and ultrasensitive method for the simultaneous detection of baicalin and baicalein, two key flavonoid from Scutellaria baicalensis, is critical, particularly in the context of traditional Chinese medicine. This study focuses on an innovative electrochemical approach, utilizing a biochar (BC)/ZIF-67/MnCo2O4 composite, which was fabricated via a combination of ultrasonic, calcination, and hydrothermal treatments. The resulting BC/ZIF-67/MnCo2O4 electrode exhibits exceptional sensitivity, with linear response ranges of 0.01 μM-400 μM. The limits of detection (LOD) for baicalein and baicalin were as low as 0.056 nM and 0.49 nM, respectively. The practical evaluations in serum and Scutellaria baicalensis demonstrate high recovery rates of 93.65 %-95.25 % for baicalein and 98.23 %-101.52 % for baicalin, with relative standard deviations below 3.99 % and 2.92 %, respectively. This study not only presents a promising technique for quality control in traditional Chinese medicine but also expands the potential applications of BC/ZIF-67/MnCo2O4 composites in electrochemical sensing, paving the way for applications in food safety monitoring and herbal medicine standardization.