Nanoelectrospray ionization coupled to a linear charge detection array ion trap spectrometer for single viral particle analysis.

This work presents the implementation of a new charge detection mass spectrometer (CDMS) design that operates in stand-alone mode, enabled by integration with nanoelectrospray ionization. More specifically, this innovative CDMS consists of a linear charge detection array ion trap spectrometer that combines an eight-tube detector array with conical electrodes. This configuration allows recording data in both transmission mode (linear array) and ion trapping mode (ConeArrayTrap) and measuring the time-of-flight (related to the mass-to-charge ratio) along with the charge of individual ions. Thus, this design supports the high-throughput metrology of viruses at the single-particle level. The devices and geometry of the instrument have been developed based on ion optics simulations. The performance of the current instrument is demonstrated using human norovirus-like particles (hNoVLP) and Adenovirus Ad(5) (hAdV5).