Simultaneous removal of nitrate, lead, and tetracycline by a fixed-biofilm reactor assembled with kapok fiber and sponge iron: Comparative analysis of operating conditions and biotic community.

In recent years, under the condition of lack of carbon source, the presence of composite micro-pollutants make the removal of nitrate seriously damaged, and to find a suitable way to solve this problem is imminent. A fixed-biofilm carrier modified by mixing sponge iron (SI) and kapok fiber (KF) combined with strain Zoogloea sp. FY6 was constructed in this study to get a fixed-biofilm reactor with merit denitrification performance. By adjusting the operation parameters, it can be concluded that when the carbon to nitrogen (C/N) ratio was 1.5, the hydraulic retention time (HRT) was 6.0 h, and the pH was 6.0, the nitrate removal efficiency (NRE) of the fixed-biofilm reactor was up to 95.4% (2.95 mg L-1 h-1). In addition, the fixed-biofilm reactor constructed in this study can remove lead (Pb2+) and tetracycline (TC) excellently in the presence of SI and Zoogloea sp. FY6, and the denitrification performance can still maintain a high level under the influence of different concentrations of Pb2+ and TC. Furthermore, the addition of SI not only removes the compound pollutants, but also protects the toxicity of the pollutant inflow in the bioreactor, and the metabolic process of microorganisms in the bioreactor also removes some of the compound pollutants. The high-throughput data showed the abundance of strain Zoogloea sp. FY6 was still the highest value under the influence of various pollutants, and the metagenomic prediction showed that the fixed-biofilm reactor had perfect denitrification process and iron redox cycle benefits. This study provides a valuable reference for sustainable utilization of natural biological resources and reduction of material costs in wastewater treatment plants (WWTPs).