[Study on quality standard for mori cortex].

OBJECTIVE

To establish a new evaluation model and compare the differences of Mori Cortex from different habitats at different harvest time.

METHODS

TLC method was used to identify sanggenon D in the sample with silica gel G plate and a mixture of chloroform-methanol-formic acid (5: 1:0. 3) as a developing solvent. UV was used for determination the centent of total flavonoids of Mori Cortex with sanggenon C as the reference substance. HPLC was applied for determination the contents of sanggenons C, D and DNJ of Mori Cortex.

RESULTS

In the TLC chromatogram, sanggenon D showed a distinct fluorescence spot under UV 365 nm with good separation. In UV, sanggenon C calibration curve showed a good linear relationship at the range of 146.8 - 734.0 microg/mL, the average recovery was 97.4% (RSD = 2.1%); For the HPLC quantitation method, sanggenons C, D and DNJ showed good linear within the scope of 700 - 4 000 microg, 500 - 3 000 microg and 4.8 - 96 microg, respectively. The average recovery of sanggenons C, D and DNJ were 98.8% (RSD = 2.6%), 99.1% (RSD = 2.2%) and 98.9% (RSD = 1.7%), respectively. The contents of index components in samples from different habitats at different harvest time were different.

CONCLUSION

The established method is reliable and accurate. It can be used for quality control of Mori Cortex.