New biotechnology · 2025
The diversity and keystone species of cucumber rhizosphere microbiome: Unveiling their role in driving cucumber growth and microbial communities.
СтатьяНазвание и аннотация: Перевод в очереди
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Yin Yaping, Khan Raja Asad Ali, Li Liping, Wu Yinggu, Li Chun, Li Yuejian, Ren Sen, Zhang Jing, Wang Weiwei, Lin Runmao, Zhang Manman, Liu Xiaojun, Hou Jumei, Liang Genyun
Аннотация
BACKGROUND: Studying the interrelationship between agroecosystems and the microbiome is essential in achieving sustainable agricultural development. In particular, the relationship between the rhizosphere microbiome and crops is critical to the health and stability of agroecosystems. This study evaluated the diversity and keystone species of the cucumber rhizosphere microbiome and investigated their contributing role in cucumber growth and microbial community regulation. A total of 108 soil samples were collected from the rhizosphere and bulk soil of cucumber crops in different locations and under different cultivation modes in a high-yield area. These samples were analyzed to study the interrelationships between different cultivation modes and crop rhizosphere microorganisms.
RESULTS: The results revealed significant regional differences in microbial communities due to microhabitat (rhizosphere and bulk soil) and geographic location. Common bacteria such as Pseudomonas, Sphingomonas, Lysobacter and Rhodanobacter were the most abundant and showed strong correlations with several other bacteria through co-occurrence networking. In addition, Flavobacterium sp. and Bacillus sp. were identified as keystone species for the rhizobia community structure. Synthetic microbial communities and pot experiments confirmed that these two keystone species could enrich the soil microbime and promote cucumber growth. Flavobacterium sp. may promote cucumber growth by regulating the rhizosphere microbial community while Bacillus sp. may influence cucumber growth by stabilizing the rhizosphere microbial biomass, further enhancing nutrient cycling efficiency and improving system stability, which in turn affected the agroecosystem.
CONCLUSIONS: Our study demonstrates that specific keystone species are important in shaping the cucumber rhizosphere microbiome and plant growth. These results provide insights into agroecosystem-microbe interrelationships and provide a potential basis for optimizing agricultural management strategies.
RESULTS: The results revealed significant regional differences in microbial communities due to microhabitat (rhizosphere and bulk soil) and geographic location. Common bacteria such as Pseudomonas, Sphingomonas, Lysobacter and Rhodanobacter were the most abundant and showed strong correlations with several other bacteria through co-occurrence networking. In addition, Flavobacterium sp. and Bacillus sp. were identified as keystone species for the rhizobia community structure. Synthetic microbial communities and pot experiments confirmed that these two keystone species could enrich the soil microbime and promote cucumber growth. Flavobacterium sp. may promote cucumber growth by regulating the rhizosphere microbial community while Bacillus sp. may influence cucumber growth by stabilizing the rhizosphere microbial biomass, further enhancing nutrient cycling efficiency and improving system stability, which in turn affected the agroecosystem.
CONCLUSIONS: Our study demonstrates that specific keystone species are important in shaping the cucumber rhizosphere microbiome and plant growth. These results provide insights into agroecosystem-microbe interrelationships and provide a potential basis for optimizing agricultural management strategies.