Science (New York, N.Y.) · 2002
Dynamics of thymocyte-stromal cell interactions visualized by two-photon microscopy.
СтатьяПоддержка исследования: вне правительства СШАПоддержка службы здравоохранения СШАНазвание и аннотация: Перевод в очереди
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Bousso Philippe, Bhakta Nirav R, Lewis Richard S, Robey Ellen
Аннотация
Thymocytes are selected to mature according to their ability to interact with self major histocompatibility complex (MHC)-peptide complexes displayed on the thymic stroma. Using two-photon microscopy, we performed real-time analysis of the cellular contacts made by developing thymocytes undergoing positive selection in a three-dimensional thymic organ culture. A large fraction of thymocytes within these cultures were highly motile. MHC recognition was found to increase the duration of thymocyte-stromal cell interactions and occurred as both long-lived cellular associations displaying stable cell-cell contacts and as shorter, highly dynamic contacts. Our results identify the diversity and dynamics of thymocyte interactions during positive selection.
Предметные рубрики MeSH
AnimalsAntigen PresentationBenzopyransCD8-Positive T-LymphocytesCell AggregationCell CommunicationCell MovementCell SizeCoculture TechniquesFluoresceinsFluorescent DyesHistocompatibility Antigens Class ILasersMiceMice, Inbred C57BLMice, TransgenicMicroscopyNaphtholsOrgan Culture TechniquesPhotonsReceptors, Antigen, T-CellRhodaminesSignal TransductionStromal CellsSuccinimidesT-Lymphocyte SubsetsT-LymphocytesThymus GlandTime Factors