Auris, nasus, larynx · 2012
Dopamine increases Na+ absorption in the Reissner's membrane of the gerbil cochlea.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Kim Chang-Hee, Kim Hye-Young, Lee Ho Sun, Choi Byung Yoon, Chang Sun O, Oh Seung-Ha, Lee Jun Ho
Аннотация
OBJECTIVE: The purpose of the present study was to investigate the effect of dopamine as a possible regulator of epithelial Na(+) channel (ENaC) in the Reissner's membrane (RM).
METHODS: RM was freshly dissected from the gerbil cochlea, and short-circuit current (Isc) was measured using the voltage-sensitive vibrating probe technique. The dopamine receptor expression was examined using immunohistochemistry.
RESULTS: The results showed that dopamine induced activation of the amiloride-sensitive Isc, but not after pre-treatment with amiloride. The D1-like receptor antagonist SCH-23390, but not the D2-like receptor antagonist sulpiride, decreased the stimulatory effect of dopamine on RM. The effect of dopamine on Na(+) transport via ENaC was still observed after blockade of the Na(+)-K(+)-ATPase by ouabain. D1 receptor immunoreactivity was observed in RM, stria vascularis and spiral ganglion.
CONCLUSION: Na(+) transport in RM is activated by dopamine possibly via D1-like receptors, and intracellular mechanisms other than cAMP-mediated pathway may be involved.
METHODS: RM was freshly dissected from the gerbil cochlea, and short-circuit current (Isc) was measured using the voltage-sensitive vibrating probe technique. The dopamine receptor expression was examined using immunohistochemistry.
RESULTS: The results showed that dopamine induced activation of the amiloride-sensitive Isc, but not after pre-treatment with amiloride. The D1-like receptor antagonist SCH-23390, but not the D2-like receptor antagonist sulpiride, decreased the stimulatory effect of dopamine on RM. The effect of dopamine on Na(+) transport via ENaC was still observed after blockade of the Na(+)-K(+)-ATPase by ouabain. D1 receptor immunoreactivity was observed in RM, stria vascularis and spiral ganglion.
CONCLUSION: Na(+) transport in RM is activated by dopamine possibly via D1-like receptors, and intracellular mechanisms other than cAMP-mediated pathway may be involved.
Предметные рубрики MeSH
8-Bromo Cyclic Adenosine MonophosphateAmilorideAnimalsBenzazepinesCochleaDopamineDopamine AgentsDopamine AntagonistsDose-Response Relationship, DrugEnzyme InhibitorsEpithelial CellsEpithelial Sodium Channel BlockersEpithelial Sodium ChannelsGerbillinaeImmunohistochemistryMembranesOuabainReceptors, DopamineSodiumSulpiride