Unravelling the composition, antioxidant, antimicrobial potential of Himalayan propolis and in vitro evaluation of its cytotoxicity against pancreatic PANC-1 cell lines.

The present study aimed to investigate the bioactive constituents, antioxidant and cytotoxic activity as well as the susceptibility of the Escherichia coli and Candida albicans to ethanolic extracts of Himalayan propolis. Phenolic and flavonoid content were quantified using FC reagent and AlCl3 methods, respectively. Free-radical scavenging activity and bioactive constituents were assessed using the DPPH and LC-MS analysis, respectively. Cytotoxicity was evaluated by using MTT assay for PANC-1 cell lines. TFC and TPC for EEP were 14.246 ± 0.08 (mg/g) QE and 9.455 ± 0.14 (mg/g) GAE respectively. EEP inhibited the growth of microbes and showed effective action establishing a positive correlation between antimicrobial activity and flavonoid content. Propolis-treated PANC-1 cell lines suppressed cell proliferation in a dose-dependent manner and exhibited positive potential. Results revealed that the promising pharmacological potential of Himalayan propolis is due to the -bioactive compounds like pinocembrin and coumarin.