The Journal of pharmacy and pharmacology · 2009
Effect of Guanxin No.2 decoction on gene expression in different areas of the myocardial infarcted heart of rats using microarray technology.
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Zeng Xiaowei, Yang Jun, Yang Xianzhe, Hong Dongsheng, Wu Limao, Yu Jun
Аннотация
OBJECTIVES: We have used microarray technology to detect the effect of Guanxin No.2 decoction on gene expression in different areas of the myocardial infarcted heart of rats.
METHODS: Male Sprague-Dawley rats (180-200 g) were randomly divided into three groups: sham-operated; coronary artery ligation; and coronary artery ligation plus administration of Guanxin No.2 decoction (10.0 g raw materials/kg per day by gavage). The experiment was carried out on day seven after ligation.
KEY FINDINGS: We found that the gene expression using microarray technology showed many differences in the border infarcted left ventricular area compared with the remote noninfarcted left ventricular area after administration of Guanxin No.2 decoction.
CONCLUSIONS: Guanxin No.2 decoction has a long history in treating ischaemic cardiomyopathy in China, but the molecular mechanism has been unclear. In this study we found that some important genes may have contributed to the cardioprotective effect of Guanxin No.2 decoction.
METHODS: Male Sprague-Dawley rats (180-200 g) were randomly divided into three groups: sham-operated; coronary artery ligation; and coronary artery ligation plus administration of Guanxin No.2 decoction (10.0 g raw materials/kg per day by gavage). The experiment was carried out on day seven after ligation.
KEY FINDINGS: We found that the gene expression using microarray technology showed many differences in the border infarcted left ventricular area compared with the remote noninfarcted left ventricular area after administration of Guanxin No.2 decoction.
CONCLUSIONS: Guanxin No.2 decoction has a long history in treating ischaemic cardiomyopathy in China, but the molecular mechanism has been unclear. In this study we found that some important genes may have contributed to the cardioprotective effect of Guanxin No.2 decoction.
Предметные рубрики MeSH
AnimalsBenzaldehydesBenzoatesBenzofuransBridged-Ring CompoundsCardiotonic AgentsCarthamus tinctoriusCatecholsChalconeCoumaric AcidsDalbergiaDrugs, Chinese HerbalGene ExpressionGene Expression ProfilingGlucosidesHeart VentriclesHydroxybenzoatesLactatesLigusticumMaleMonoterpenesMyocardial InfarctionOligonucleotide Array Sequence AnalysisPaeoniaPlant ExtractsQuinonesRatsRats, Sprague-DawleyReverse Transcriptase Polymerase Chain ReactionSalvia miltiorrhizaGlycosidesDepsides