Revista da Sociedade Brasileira de Medicina Tropical · 2019
Fragment detection of Coleopteran and Triatomine insects in experimentally contaminated acai pulp and sugarcane juice.
Сравнительное исследованиеСтатьяНазвание и аннотация: Перевод пока не подготовлен
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Mattos Elaine Cristina de, Marciano Maria Aparecida Moraes, Daros Vilma Dos Santos Menezes Gaiotto, Faccini Cristiane Castro, Lourenço Angela Maria, Pereira-Chioccola Vera Lucia
Аннотация
INTRODUCTION: Oral transmission of acute Chagas disease is an emerging public health concern. This study aimed to detect insect fragments in experimentally contaminated food, by comparing triatomines with other insects.
METHODS: Food samples were experimentally contaminated with insects, processed to recover their fragments by light filth, and analyzed by microscopy and Polymerase Chain Reaction (PCR).
RESULTS: Morphological differences between coleopteran and triatomine insects were observed in microscopic images. PCR was efficient in amplifying Triatominae DNA in the experimentally contaminated food.
CONCLUSIONS: This methodology could be utilized by food analysts to identify possible insect contamination in food samples.
METHODS: Food samples were experimentally contaminated with insects, processed to recover their fragments by light filth, and analyzed by microscopy and Polymerase Chain Reaction (PCR).
RESULTS: Morphological differences between coleopteran and triatomine insects were observed in microscopic images. PCR was efficient in amplifying Triatominae DNA in the experimentally contaminated food.
CONCLUSIONS: This methodology could be utilized by food analysts to identify possible insect contamination in food samples.