Fragment detection of Coleopteran and Triatomine insects in experimentally contaminated acai pulp and sugarcane juice.

INTRODUCTION

Oral transmission of acute Chagas disease is an emerging public health concern. This study aimed to detect insect fragments in experimentally contaminated food, by comparing triatomines with other insects.

METHODS

Food samples were experimentally contaminated with insects, processed to recover their fragments by light filth, and analyzed by microscopy and Polymerase Chain Reaction (PCR).

RESULTS

Morphological differences between coleopteran and triatomine insects were observed in microscopic images. PCR was efficient in amplifying Triatominae DNA in the experimentally contaminated food.

CONCLUSIONS

This methodology could be utilized by food analysts to identify possible insect contamination in food samples.