Open veterinary journal · 2025
Effect of fermented red bean extract (Phaseolus vulgaris L.) on spermatogenic cells in mice exposed to smoke.
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Novanto Elias Setyo, Khairullah Aswin Rafif, Budiarto Budiarto, Lestari Tita Damayanti, Madyawati Sri Pantja, Hernawati Tatik, Rachmawati Kadek, Mustofa Imam, Rasad Siti Darodjah, Dawood Ahmed Qasim, Riady Ginta, Wasito Wasito, Ahmad Riza Zainuddin, Pratama Bima Putra, Chairuman Novia
Аннотация
BACKGROUND: Red beans are rich in bioactive compounds, such as flavonoids, anthocyanins, tannins, and polyphenols, which have high antioxidant activity and are believed to provide protection against testicular tissue exposure to toxic substances, including cigarette smoke.
AIM: This study aims to scientifically analyze the effect of fermented red bean extract on the number of spermatogenic cells in male mice exposed to cigarette smoke.
METHODS: This experimental study included 25 mice. Group K (-) was administered 0.5 ml of 1% CMC-Na, group K (+) was administered 0.5 ml of 1% CMC-Na and exposed to cigarette smoke, and groups P1, P2, and P3 were administered fermented red bean extract (Phaseolus vulgaris L.) at doses of 26 mg/kgBW, 52 mg/kgBW, and 104 mg/kgBW, respectively, and exposed to cigarette smoke. Each group was given one cigarette per day for 36 days. Testicular histopathology preparations were made with hematoxylin and eosin staining and continued with spermatogenic cell counting. Data were analyzed using the one-way analysis of variance and continued with Duncan's test (p < 0.05).
RESULTS: The results showed that the K(-) group had the highest number of spermatogenic cells, while the K(+) group had the lowest number of spermatogenic cells. The P2 group was the most effective method in maintaining the number of cells because the P2 group had a good number of cells even with a lower dose compared to the P3 group.
CONCLUSION: This study concluded that the dose of 52 mg/kgBW has the best potential. Fermented red bean extract contains isoflavones that act as free radical scavengers by donating electrons to reactive oxygen species to stabilize the molecule.
AIM: This study aims to scientifically analyze the effect of fermented red bean extract on the number of spermatogenic cells in male mice exposed to cigarette smoke.
METHODS: This experimental study included 25 mice. Group K (-) was administered 0.5 ml of 1% CMC-Na, group K (+) was administered 0.5 ml of 1% CMC-Na and exposed to cigarette smoke, and groups P1, P2, and P3 were administered fermented red bean extract (Phaseolus vulgaris L.) at doses of 26 mg/kgBW, 52 mg/kgBW, and 104 mg/kgBW, respectively, and exposed to cigarette smoke. Each group was given one cigarette per day for 36 days. Testicular histopathology preparations were made with hematoxylin and eosin staining and continued with spermatogenic cell counting. Data were analyzed using the one-way analysis of variance and continued with Duncan's test (p < 0.05).
RESULTS: The results showed that the K(-) group had the highest number of spermatogenic cells, while the K(+) group had the lowest number of spermatogenic cells. The P2 group was the most effective method in maintaining the number of cells because the P2 group had a good number of cells even with a lower dose compared to the P3 group.
CONCLUSION: This study concluded that the dose of 52 mg/kgBW has the best potential. Fermented red bean extract contains isoflavones that act as free radical scavengers by donating electrons to reactive oxygen species to stabilize the molecule.