Hua xi kou qiang yi xue za zhi = Huaxi kouqiang yixue zazhi = West China journal of stomatology · 2000
[Changes of cytokines secreted by human oral mucosa keratinocytes from oral submucous fibrosis in vitro].
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Feng Y, Ling T
Аннотация
OBJECTIVE: To assess the significance of oral mucosa keratinocytes (KCs) and cytokines in the development of oral submucous fibrosis (OSF).
METHODS: KCs were obtained and cultured from the buccal mucosa of healthy subjects who did not chew areca nut and patients with OSF who chewed areca nut. Then radioimmunoassay and enzyme-linked immunosorbent assays were employed, and endothelin (ET) and transforming growth factor beta 1 (TGF beta 1) were quantified in culture supernatants.
RESULTS: The mean levels of ET and TGF beta 1 secreted by OSF-KC were 96.983 +/- 17.802 pg/ml and 4661.641 +/- 783.893 pg/ml respectively, which were higher than these secreted by NM-KC (P < 0.05); There was a positive correlation between the levels of ET and TGF beta 1 secreted by OSF-KC.
CONCLUSION: External stimuli such as areca nut components may induce the development of OSF by activating and stimulating the keratinocytes to secret a series of cytokines, including ET and TGF beta 1. It is speculated that ET and TGF beta 1 may be the contributing factors in the pathological features noted in OSF.
METHODS: KCs were obtained and cultured from the buccal mucosa of healthy subjects who did not chew areca nut and patients with OSF who chewed areca nut. Then radioimmunoassay and enzyme-linked immunosorbent assays were employed, and endothelin (ET) and transforming growth factor beta 1 (TGF beta 1) were quantified in culture supernatants.
RESULTS: The mean levels of ET and TGF beta 1 secreted by OSF-KC were 96.983 +/- 17.802 pg/ml and 4661.641 +/- 783.893 pg/ml respectively, which were higher than these secreted by NM-KC (P < 0.05); There was a positive correlation between the levels of ET and TGF beta 1 secreted by OSF-KC.
CONCLUSION: External stimuli such as areca nut components may induce the development of OSF by activating and stimulating the keratinocytes to secret a series of cytokines, including ET and TGF beta 1. It is speculated that ET and TGF beta 1 may be the contributing factors in the pathological features noted in OSF.