Forebrain Lineage Factor DMRTA2 Is Not Required for Maintenance of H3G34-Mutant Paediatric High-Grade Gliomas.

Developmental transcriptional programs are increasingly recognized as key drivers of paediatric gliomagenesis. Here, we unexpectedly find that the forebrain-specific doublesex- and mab-3-related transcription factor a2 (DMRTA2), despite being highly expressed in H3G34-mutant diffuse hemispheric glioma (DHG-H3G34) and implicated in tumour initiation, is not required for the maintenance of established patient-derived tumour cells. These findings support a stage-specific framework in which transcriptional dependencies are dynamically rewired during tumour progression in paediatric brain tumours.