The Laryngoscope · 2014
Squamous cell carcinoma of the head and neck outside the oropharynx is rarely human papillomavirus related.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Upile Navdeep S, Shaw Richard J, Jones Terry M, Goodyear Paul, Liloglou Triantafillos, Risk Janet M, Boyd Mark T, Sheard Jon, Sloan Phil, Robinson Max, Schache Andrew G
Аннотация
OBJECTIVES/HYPOTHESIS: The incidence of human papillomavirus (HPV)-driven disease beyond the oropharynx varies greatly in the reported literature.
STUDY DESIGN: Case series.
METHODS: Two hundred twenty-one samples were strictly classified to the subsites of oral cavity, larynx, or hypopharynx at the time of primary surgery. Formalin-fixed paraffin-embedded samples were subjected to a validated, tiered, diagnostic algorithm of p16 immunohistochemistry, high-risk HPV in situ hybridization, and quantitative polymerase chain reaction for HPV E6 DNA. An additional 60 oropharyngeal cases acted as an internal biological control.
RESULTS: An incidence of 4% of HPV-driven cases was observed across the subsites outside the oropharynx compared to 70% of tumors confined within it.
CONCLUSIONS: This is the first reporting of a broad range of nonoropharyngeal HPV rates using this validated diagnostic algorithm. It remains unclear whether patients with HPV-driven disease originating outside the oropharynx enjoy the same survival advantage apparent in those patients with oropharyngeal squamous cell carcinomas.
LEVEL OF EVIDENCE: 4 Laryngoscope, 124:2739-2744, 2014.
STUDY DESIGN: Case series.
METHODS: Two hundred twenty-one samples were strictly classified to the subsites of oral cavity, larynx, or hypopharynx at the time of primary surgery. Formalin-fixed paraffin-embedded samples were subjected to a validated, tiered, diagnostic algorithm of p16 immunohistochemistry, high-risk HPV in situ hybridization, and quantitative polymerase chain reaction for HPV E6 DNA. An additional 60 oropharyngeal cases acted as an internal biological control.
RESULTS: An incidence of 4% of HPV-driven cases was observed across the subsites outside the oropharynx compared to 70% of tumors confined within it.
CONCLUSIONS: This is the first reporting of a broad range of nonoropharyngeal HPV rates using this validated diagnostic algorithm. It remains unclear whether patients with HPV-driven disease originating outside the oropharynx enjoy the same survival advantage apparent in those patients with oropharyngeal squamous cell carcinomas.
LEVEL OF EVIDENCE: 4 Laryngoscope, 124:2739-2744, 2014.
Предметные рубрики MeSH
Biomarkers, TumorCarcinoma, Squamous CellDNA, ViralDiagnosis, DifferentialFemaleFollow-Up StudiesHead and Neck NeoplasmsHumansImmunohistochemistryIn Situ HybridizationMaleMiddle AgedOropharynxPapillomaviridaePapillomavirus InfectionsPolymerase Chain ReactionReal-Time Polymerase Chain ReactionRetrospective StudiesSquamous Cell Carcinoma of Head and Neck