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The quantification and mRNA expression levels of cochlear synapses in C57BL/6j mice following repeated exposure to noise.
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Qian Minfei, Yao Zhuowei, Wang Qixuan, Zhou Yaqi, Huang Zhiwu, Li Jiping
Аннотация
BACKGROUND: Noise-induced cochlear synaptopathy has recently emerged as a focus in hearing research.
PURPOSE: This study aimed to examine the impact of repeated noise exposure on the quantification and mRNA expression levels of cochlear synapses.
METHODS: Measurements were conducted at baseline, 1 day, and 14 days post-exposure to 88 or 97 dB SPL noise (2 h/day for 7 days, frequency range 2-20 kHz). Auditory brainstem responses (ABRs), immunofluorescence and quantitative real-time PCR (qRT-PCR) were used to examine the results.
RESULTS: 1. Exposure to 88 dB SPL caused minimal changes in ABRs, ribbon morphology and medial olivocochlear (MOC) efferent synapses; elevation of synaptophysin(SYP) and α9α10 nAchR mRNA levels were observed. 2. Exposure to 97 dB SPL caused threshold shift and synaptopathy of ribbon and MOC; downregulation of α10nAchR, SYP and ctbp2 mRNA levels were observed.
CONCLUSION: Noise-induced cochlear synaptic degeneration involves both afferent and efferent synaptopathy.
PURPOSE: This study aimed to examine the impact of repeated noise exposure on the quantification and mRNA expression levels of cochlear synapses.
METHODS: Measurements were conducted at baseline, 1 day, and 14 days post-exposure to 88 or 97 dB SPL noise (2 h/day for 7 days, frequency range 2-20 kHz). Auditory brainstem responses (ABRs), immunofluorescence and quantitative real-time PCR (qRT-PCR) were used to examine the results.
RESULTS: 1. Exposure to 88 dB SPL caused minimal changes in ABRs, ribbon morphology and medial olivocochlear (MOC) efferent synapses; elevation of synaptophysin(SYP) and α9α10 nAchR mRNA levels were observed. 2. Exposure to 97 dB SPL caused threshold shift and synaptopathy of ribbon and MOC; downregulation of α10nAchR, SYP and ctbp2 mRNA levels were observed.
CONCLUSION: Noise-induced cochlear synaptic degeneration involves both afferent and efferent synaptopathy.