The Kobe journal of medical sciences · 2017
New, Improved Version of the mCOP-PCR Screening System for Detection of Spinal Muscular Atrophy Gene (SMN1) Deletion.
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Shinohara Masakazu, Ar Rochmah Mawaddah, Nakanishi Kenta, Harahap Nur Imma Fatimah, Niba Emma Tabe Eko, Saito Toshio, Saito Kayoko, Takeuchi Atsuko, Bouike Yoshihiro, Nishio Hisahide
Аннотация
BACKGROUND: Spinal muscular atrophy (SMA) is a frequent autosomal recessive disorder, characterized by lower motor neuron loss in the spinal cord. More than 95% of SMA patients show homozygous survival motor neuron 1 (SMN1) deletion. We previously developed a screening system for SMN1 deletion based on a modified competitive oligonucleotide priming-PCR (mCOP-PCR) technique. However, non-specific amplification products were observed with mCOP-PCR, which might lead to erroneous interpretation of the screening results.
AIM: To establish an improved version of the mCOP-PCR screening system without non-specific amplification.
METHODS: DNA samples were assayed using a new version of the mCOP-PCR screening system. DNA samples had already been genotyped by PCR-restriction fragment length polymorphism (PCR-RFLP), showing the presence or absence of SMN1 exon 7. The new mCOP-PCR method contained a targeted pre-amplification step of the region, including an SMN1-specific nucleotide, prior to the mCOP-PCR step. mCOP-PCR products were electrophoresed on agarose gels.
RESULTS: No non-specific amplification products were detected in electrophoresis gels with the new mCOP-PCR screening system.
CONCLUSION: An additional targeted pre-amplification step eliminated non-specific amplification from mCOP-PCR screening.
AIM: To establish an improved version of the mCOP-PCR screening system without non-specific amplification.
METHODS: DNA samples were assayed using a new version of the mCOP-PCR screening system. DNA samples had already been genotyped by PCR-restriction fragment length polymorphism (PCR-RFLP), showing the presence or absence of SMN1 exon 7. The new mCOP-PCR method contained a targeted pre-amplification step of the region, including an SMN1-specific nucleotide, prior to the mCOP-PCR step. mCOP-PCR products were electrophoresed on agarose gels.
RESULTS: No non-specific amplification products were detected in electrophoresis gels with the new mCOP-PCR screening system.
CONCLUSION: An additional targeted pre-amplification step eliminated non-specific amplification from mCOP-PCR screening.