The Journal of antimicrobial chemotherapy · 2008
Vibrio cholerae O1 from Accra, Ghana carrying a class 2 integron and the SXT element.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
Обработка статьи · этапы, ошибки и управление
Загрузка состояния…
Ошибки и ограничения
История · было → стало
Состояние обновляется каждые 5 секунд, пока блок открыт. Процент показывается только там, где известен объём работы. Пауза останавливает продолжение этапа; текущий запрос к источнику может завершиться.
Opintan Japheth A, Newman Mercy J, Nsiah-Poodoh Owusu Agyemang, Okeke Iruka N
Аннотация
OBJECTIVES: Vibrio cholerae O1 from a 2006 outbreak in Accra were commonly resistant to multiple antimicrobials and, in particular, to trimethoprim/sulfamethoxazole, drugs commonly used in the treatment of cholera. We sought to determine the genetic basis for trimethoprim/sulfamethoxazole resistance in outbreak isolates.
METHODS: Twenty-seven isolates from the outbreak were screened by PCR and sequencing for class 1 and 2 integrons and for the SXT element.
RESULTS: Twenty-one of the 27 isolates examined, all from the Accra metropolitan area, carried both SXT, an integrated chromosomal element, and a class 2 integron bearing dfrA1, sat and aadA1 cassettes. All these isolates had identical random amplification of polymorphic DNA profiles and two of them also carried a class 1 integron.
CONCLUSIONS: Most strains characterized carried multiple elements conferring resistance to trimethoprim. This suggests that trimethoprim/sulfamethoxazole should not be used empirically in cholera treatment.
METHODS: Twenty-seven isolates from the outbreak were screened by PCR and sequencing for class 1 and 2 integrons and for the SXT element.
RESULTS: Twenty-one of the 27 isolates examined, all from the Accra metropolitan area, carried both SXT, an integrated chromosomal element, and a class 2 integron bearing dfrA1, sat and aadA1 cassettes. All these isolates had identical random amplification of polymorphic DNA profiles and two of them also carried a class 1 integron.
CONCLUSIONS: Most strains characterized carried multiple elements conferring resistance to trimethoprim. This suggests that trimethoprim/sulfamethoxazole should not be used empirically in cholera treatment.
Предметные рубрики MeSH
AdultAnti-Bacterial AgentsBacterial ProteinsChildChild, PreschoolCholeraCluster AnalysisDNA FingerprintingDNA, BacterialDisease OutbreaksDrug Resistance, BacterialFemaleGhanaHumansIntegronsInterspersed Repetitive SequencesMaleMicrobial Sensitivity TestsMiddle AgedMolecular EpidemiologyRandom Amplified Polymorphic DNA TechniqueTrimethoprim, Sulfamethoxazole Drug CombinationVibrio cholerae O1