The EMBO journal · 2009
Pre-fibrillar alpha-synuclein variants with impaired beta-structure increase neurotoxicity in Parkinson's disease models.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод пока не подготовлен
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Karpinar Damla Pinar, Balija Madhu Babu Gajula, Kügler Sebastian, Opazo Felipe, Rezaei-Ghaleh Nasrollah, Wender Nora, Kim Hai-Young, Taschenberger Grit, Falkenburger Björn H, Heise Henrike, Kumar Ashutosh, Riedel Dietmar, Fichtner Lars, Voigt Aaron, Braus Gerhard H, Giller Karin, Becker Stefan, Herzig Alf, Baldus Marc, Jäckle Herbert, Eimer Stefan, Schulz Jörg B, Griesinger Christian, Zweckstetter Markus
Аннотация
The relation of alpha-synuclein (alphaS) aggregation to Parkinson's disease (PD) has long been recognized, but the mechanism of toxicity, the pathogenic species and its molecular properties are yet to be identified. To obtain insight into the function different aggregated alphaS species have in neurotoxicity in vivo, we generated alphaS variants by a structure-based rational design. Biophysical analysis revealed that the alphaS mutants have a reduced fibrillization propensity, but form increased amounts of soluble oligomers. To assess their biological response in vivo, we studied the effects of the biophysically defined pre-fibrillar alphaS mutants after expression in tissue culture cells, in mammalian neurons and in PD model organisms, such as Caenorhabditis elegans and Drosophila melanogaster. The results show a striking correlation between alphaS aggregates with impaired beta-structure, neuronal toxicity and behavioural defects, and they establish a tight link between the biophysical properties of multimeric alphaS species and their in vivo function.