Turkiye parazitolojii dergisi · 2025
An Investigation of Microsporidia Species in Humans Living in Düzce and Bolu Provinces of Türkiye.
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Çakmak Mücahit, Ayaz Erol, Öksüz Şükrü, Dönmez Gökboğa Betül, Ege Nagihan
Аннотация
OBJECTIVE: The literature about microsporidia clearly shows that the prevalence of microsporidia varies at different rates depending on the sampling status and region in the studies. However, the majority of studies have been conducted in immunocompromised individuals. It is vital to determine the prevalence of pathogens in society in order to protect immunocompromised individuals from opportunistic pathogens. Consequently, the present study investigated stool samples from Düzce and Bolu provinces for microsporidia, irrespective of disease group.
METHODS: A study of 400 stool samples from Düzce and Bolu was performed. The samples were first stained with trichrome and examined under a microscope at 100x magnification. DNA was then isolated, amplified with polymerase chain reaction primers, and the samples were identified. The relationship between microsporidia presence and age, sex, and diarrheal status was statistically investigated.
RESULTS: Following microscopic examination, microsporidia spores were detected in 25 of the 400 (6.25%). Microsporidia spores were detected in 6.6% (14/212) of the Düzce samples and 5.8% (11/188) of the Bolu samples. Consequently, molecular analysis revealed the presence of microsporidia in 49 of the 400 (12.25%). The positivity rates were found to be 11.3% (24/212) for Düzce and 13.3% (25/188) for Bolu. The spores detected in this study were identified as belonging to the species Enterocytozoon bieneusi. A statistical analysis was conducted, revealing no significant association between the presence of the pathogen and diarrhea complaints, gender, or age.
CONCLUSION: It is seen that the presence of microsporidia in our study is at a level similar to rates reported in immunocompetent individuals around the world. Additionally, a correlation between the presence of microsporidia and complaints has been reported, especially in immunocompromised individuals, but no relationship has been determined with complaints in this study. Furthermore, the present study demonstrated that neither age nor gender exhibited any correlation with the presence of microsporidia.
METHODS: A study of 400 stool samples from Düzce and Bolu was performed. The samples were first stained with trichrome and examined under a microscope at 100x magnification. DNA was then isolated, amplified with polymerase chain reaction primers, and the samples were identified. The relationship between microsporidia presence and age, sex, and diarrheal status was statistically investigated.
RESULTS: Following microscopic examination, microsporidia spores were detected in 25 of the 400 (6.25%). Microsporidia spores were detected in 6.6% (14/212) of the Düzce samples and 5.8% (11/188) of the Bolu samples. Consequently, molecular analysis revealed the presence of microsporidia in 49 of the 400 (12.25%). The positivity rates were found to be 11.3% (24/212) for Düzce and 13.3% (25/188) for Bolu. The spores detected in this study were identified as belonging to the species Enterocytozoon bieneusi. A statistical analysis was conducted, revealing no significant association between the presence of the pathogen and diarrhea complaints, gender, or age.
CONCLUSION: It is seen that the presence of microsporidia in our study is at a level similar to rates reported in immunocompetent individuals around the world. Additionally, a correlation between the presence of microsporidia and complaints has been reported, especially in immunocompromised individuals, but no relationship has been determined with complaints in this study. Furthermore, the present study demonstrated that neither age nor gender exhibited any correlation with the presence of microsporidia.