An autoradiographic study of cochlea and organs of guinea pigs after intraperitoneal injection of 125I-labelled bFGF.

CONCLUSION

The results of our research indicate that 125I-labelled basic fibroblast growth factor (125I-bFGF) has some difficulty in getting across the blood-labyrinth barrier (BLB). Therefore, it is necessary to carry out further study on the route of bFGF administration.

OBJECTIVE

To observe whether bFGF could pass through the BLB after intraperitoneal injection and to establish an experimental basis for its clinical applications.

METHODS

Thirty guinea pigs were divided into three equal groups. Animals in group 1 were administered 125I-bFGF, while animals in groups 2 and 3 were given 125I or saline solution, respectively, via intraperitoneal injection. The animals were sacrificed 2 h later and samples of blood, brain, cochlea, thyroid gland, heart, liver, and kidney were collected and weighed. A radioimmunoassay analyzer was employed to count the CPM values of each sample, and autoradiography was performed on section samples of both cochleae.

RESULTS

The CPM value of organ samples in the 125I group was higher than that in other groups, and radioactive grain was observed in cochlear samples of this group. In the 125I-bFGF group, blood demonstrated the highest CPM value, while cochlea and brain demonstrated the lowest CPM value, with no radioactive grain observed in cochlear samples.