The Pediatric infectious disease journal · 2026
Small Epidemic Outbreak of Norovirus in the Pediatric Department of Brescia Civic Hospital (Northern Italy): Genomic Characterization and Phylogenetic Analysis.
СтатьяНазвание и аннотация: Перевод в очереди
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Pellegrino Michele, Bertelli Anna, Dotta Laura, Roversi Sara, Marsico Stefania, Badolato Raffaele, Caccuri Francesca, Messali Serena
Аннотация
BACKGROUND: Human noroviruses are a leading cause of acute gastroenteritis worldwide, with GII.4 Sydney 2012 [P16], being the predominant circulating genotype in Italy in recent years. Outbreaks in pediatric hospital wards represent a major public health concern.
METHODS: Eight stool samples collected between November 2023 and February 2024 at Brescia Civic Hospital were analyzed by real-time polymerase chain reaction, sequence-independent single-primer amplification and next-generation sequencing.
RESULTS: All isolates belonged to GII.4 Sydney 2012 [P16] genotype. Five isolates from December 2023 clustered tightly together, confirming a nosocomial outbreak with near-complete sequence identity. Isolates from November 2023, January 2024 and February 2024 were phylogenetically distinct, with 1 isolate (January 2024) clustering in a completely separated tree portion, carrying 30 amino acid substitutions and 237 synonymous mutations.
CONCLUSIONS: Our analysis confirmed a nosocomial outbreak and underscored the substantial intra-genotypic diversity of GII.4 Sydney 2012 [P16] genotype. These findings highlight the importance of rapid diagnosis, infection control measures and continuous genomic surveillance to track the evolution and global circulation of epidemic noroviruses.
METHODS: Eight stool samples collected between November 2023 and February 2024 at Brescia Civic Hospital were analyzed by real-time polymerase chain reaction, sequence-independent single-primer amplification and next-generation sequencing.
RESULTS: All isolates belonged to GII.4 Sydney 2012 [P16] genotype. Five isolates from December 2023 clustered tightly together, confirming a nosocomial outbreak with near-complete sequence identity. Isolates from November 2023, January 2024 and February 2024 were phylogenetically distinct, with 1 isolate (January 2024) clustering in a completely separated tree portion, carrying 30 amino acid substitutions and 237 synonymous mutations.
CONCLUSIONS: Our analysis confirmed a nosocomial outbreak and underscored the substantial intra-genotypic diversity of GII.4 Sydney 2012 [P16] genotype. These findings highlight the importance of rapid diagnosis, infection control measures and continuous genomic surveillance to track the evolution and global circulation of epidemic noroviruses.