The Journal of veterinary medical science · 2024
Effects of exposure to the neonicotinoid pesticide clothianidin on α-defensin secretion and gut microbiota in mice.
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Yonoichi Sakura, Hara Yukako, Ishida Yuya, Shoda Asuka, Kimura Mako, Murata Midori, Nunobiki Sarika, Ito Makiko, Yoshimoto Ayano, Mantani Youhei, Yokoyama Toshifumi, Hirano Tetsushi, Ikenaka Yoshinori, Yokoi Yuki, Ayabe Tokiyoshi, Nakamura Kiminori, Hoshi Nobuhiko
Аннотация
The mechanism by which the neonicotinoid pesticide clothianidin (CLO) disrupts the intestinal microbiota of experimental animals is unknown. We focused on α-defensins, which are regulators of the intestinal microbiota. Subchronic exposure to CLO induced dysbiosis and reduced short-chain fatty acid-producing bacteria in the intestinal microbiota of mice. Levels of cryptdin-1 (Crp1, a major α-defensin in mice) in feces and cecal contents were lower in the CLO-exposed groups than in control. In Crp1 immunostaining, Paneth cells in the jejunum and ileum of the no-observed-adverse-effect-level CLO-exposed group showed a stronger positive signal than control, likely due to the suppression of Crp1 release. Our results showed that CLO exposure suppresses α-defensin secretion from Paneth cells as part of the mechanism underlying CLO-induced dysbiosis.