Fertility and sterility · 2012
Studying nonobstructive azoospermia in cystinosis: histologic examination of testes and epididymis and sperm analysis in a Ctns⁻/⁻ mouse model.
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Besouw Martine T P, van Pelt Ans M M, Gaide Chevronnay Héloïse P, Courtoy Pierre J, Pastore Anna, Goossens Ellen, Devuyst Olivier, Antignac Corinne, Levtchenko Elena N
Аннотация
OBJECTIVE: To study the pathogenesis of male infertility in cystinosis due to nonobstructive azoospermia, using a Ctns(-/-) mouse model.
DESIGN: Observational case-control study.
SETTING: Academic research laboratory.
ANIMAL(S): Male C57BL/6 Ctns(-/-) mice were compared with C57BL/6 wild-type (wt) mice.
INTERVENTION(S): None.
MAIN OUTCOME MEASURE(S): Fertility was studied using litter size (n = 3 vs. n = 2). After animals were sacrificed, testes, epididymis, and vas deferens were removed for testicular cystine measurements (n = 5 vs. n = 6), histologic studies (n = 3 vs. n = 3), and sperm analysis (n = 3 vs. n = 3).
RESULT(S): Mean testicular cystine content was significantly higher in Ctns(-/-) mice compared with wt mice (26.6 ± 1.22 vs. 0.1 ± 0.01 nmol cystine/mg protein). Testes of Ctns(-/-) mice had lower weight compared with wt mice (0.096 ± 0.009 g vs. 0.112 ± 0.004 g), but mice fertility was similar (litter size 6.6 ± 1.4 vs. 6.3 ± 2.6 pups). Neither histologic nor sperm abnormalities were found.
CONCLUSION(S): The Ctns(-/-) mouse model generated on C57BL/6 background is not suitable for clarifying the pathogenesis of male infertility in cystinosis. The etiology of nonobstructive azoospermia in these patients remains unclear.
DESIGN: Observational case-control study.
SETTING: Academic research laboratory.
ANIMAL(S): Male C57BL/6 Ctns(-/-) mice were compared with C57BL/6 wild-type (wt) mice.
INTERVENTION(S): None.
MAIN OUTCOME MEASURE(S): Fertility was studied using litter size (n = 3 vs. n = 2). After animals were sacrificed, testes, epididymis, and vas deferens were removed for testicular cystine measurements (n = 5 vs. n = 6), histologic studies (n = 3 vs. n = 3), and sperm analysis (n = 3 vs. n = 3).
RESULT(S): Mean testicular cystine content was significantly higher in Ctns(-/-) mice compared with wt mice (26.6 ± 1.22 vs. 0.1 ± 0.01 nmol cystine/mg protein). Testes of Ctns(-/-) mice had lower weight compared with wt mice (0.096 ± 0.009 g vs. 0.112 ± 0.004 g), but mice fertility was similar (litter size 6.6 ± 1.4 vs. 6.3 ± 2.6 pups). Neither histologic nor sperm abnormalities were found.
CONCLUSION(S): The Ctns(-/-) mouse model generated on C57BL/6 background is not suitable for clarifying the pathogenesis of male infertility in cystinosis. The etiology of nonobstructive azoospermia in these patients remains unclear.