De novo synthesis of GAP-43: in situ hybridization histochemistry and light and electron microscopy immunocytochemical studies in regenerating motor neurons of cranial nerve nuclei in the rat brain.

In order to investigate the modulation of the synthesis and the subcellular localization of the growth associated protein GAP-43 in neuronal cell bodies we have taken advantage of the well known regenerative properties of axotomized motor neurons of the facial and hypoglossal nuclei. Alterations in the levels of GAP-43 mRNA containing cells were studied by in situ hybridization histochemistry. The protein localization was examined using immunohistochemistry at the light and electron microscopic levels. Neurons from the control side showed undetectable levels of both GAP-43-like immunoreactivity and GAP-43 mRNA levels. Whereas axotomized neurons exhibited a marked increase in GAP-43 mRNA levels and in GAP-43-like immunoreactivity. Three to 50 days after axotomy, motor neurons ipsilateral to the lesion displayed a dense reticular or filamentous perinuclear distribution of the immunoreactivity in somata and proximal dendritic processes, corresponding to the location of the Golgi apparatus in these neurons. At the electron microscopic level the immunoreactivity was located in the cisternae of the Golgi complex and found to be associated with trans-side vesicles of these complexes. The myelinated fibers of the transectomized facial nerve also presented an intense GAP-43-like immunoreactivity. Twenty-one days after the axotomy a decay in the number of immunostained neurons and in the intensity of immunolabeled somata was observed. Our study reveals a rapid induction of GAP-43 mRNA and protein after axotomy. The localization of the newly synthesized GAP-43-like immunoreactivity to the Golgi apparatus seen in the present work suggests an early association of this protein with newly formed membranes prior to transport toward the terminals through the axons.