Biological Characteristics and Domestication Cultivation of the Medicinal Mushroom Inocutis levis (Agaricomycetes).

This study investigated the biological characteristics and domestication cultivation of Inocutis levis through single factor and orthogonal experiments. Single factor experiment showed that the most suitable carbon source for the growth of the mycelia of I. levis was mannitol, and the nitrogen source was yeast extract. The incubation temperature was 27-31°C, the liquid volume was 80-160 mL, the pH was 4.5-7.5, the rotation speed was 190-210 rpm, and the inoculation volume was 40 mL. The orthogonal experiments showed that the optimal medium formula and cultivation conditions for liquid fermentation of mycelia were as follows: adding mannitol 20 g/L, yeast extract 2 g/L in the basic formula of 20% potato, 0.15% magnesium sulfate, and 0.1% KH2PO4, pH 5.5, liquid loading volume 120 mL, incubation temperature of 30°C, and after standing for 2 d, shaking for 7 d at 190 rpm; the optimal cultivation formula was: 50% sawdust, 20% wheat bran, 20% corncob, and 7% corn stover, 1% soybean powder, 1% lime, and 1% gypsum were used, and the biological efficiency reached 69.3% . This study will lay a theoretical foundation for the production of active substances and industrial cultivation through liquid deep fermentation of I. levis.