Journal de mycologie medicale · 2017
Preparations based on minerals extracts of Calicotome villosa roots and bovine butyrate matter: Evaluation in vitro of their antifungal activity.
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Barhouchi B, Aouadi S, Abdi A
Аннотация
OBJECTIVE: The use of preparations based on minerals extracts of Calicotome villosa and butter is born from the misuse of drugs without specific microbiological analyzes. Seventeen different preparations were performed. The antibacterial and antifungal activities were determined on five bacteria and two fungi strains respectively.
MATERIAL AND METHODS: C. villosa ashes are obtained by incineration of roots plant at 498°C for 4hours. They are analyzed to determine the shape of the particles and the mineral constituents by scanning electronic microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX) techniques respectively. The effectiveness of preparations or tablets is measured in solid medium. It allows to measure the diameter of the inhibition zone for the antibacterial activity as well as the diameter of mycelia growth and the critical values (MIC, MFC, IC50 and IC90) for the antifungal activity. Finally, the results are compared to the activity of a commercial positive control aiming to give value of the observed activity.
RESULTS: SEM observations reveal the presence of nanoparticles agglomerated with size of about 50nm. The EDX analyzes indicate the presence of Fe, Na, Al, Mg, Si, K, Ca, O2 and C. Among all the results, the preparation (Bs+A) or (Bsd+A) can completely inhibit the growth of two fungal pathogens. The activity of the preparation is faced with the activity of the synthetic fungicide nystatin.
CONCLUSION: The efficacy of the preparation (Bs+A) or (Bsd+A) is higher than that of nystatin against Aspergillus sp. and Fusarium sp. The preparation could serve as natural antifungal for the pharmaceutical industry.
MATERIAL AND METHODS: C. villosa ashes are obtained by incineration of roots plant at 498°C for 4hours. They are analyzed to determine the shape of the particles and the mineral constituents by scanning electronic microscopy (SEM) and energy dispersive X-ray spectroscopy (EDX) techniques respectively. The effectiveness of preparations or tablets is measured in solid medium. It allows to measure the diameter of the inhibition zone for the antibacterial activity as well as the diameter of mycelia growth and the critical values (MIC, MFC, IC50 and IC90) for the antifungal activity. Finally, the results are compared to the activity of a commercial positive control aiming to give value of the observed activity.
RESULTS: SEM observations reveal the presence of nanoparticles agglomerated with size of about 50nm. The EDX analyzes indicate the presence of Fe, Na, Al, Mg, Si, K, Ca, O2 and C. Among all the results, the preparation (Bs+A) or (Bsd+A) can completely inhibit the growth of two fungal pathogens. The activity of the preparation is faced with the activity of the synthetic fungicide nystatin.
CONCLUSION: The efficacy of the preparation (Bs+A) or (Bsd+A) is higher than that of nystatin against Aspergillus sp. and Fusarium sp. The preparation could serve as natural antifungal for the pharmaceutical industry.