Scandinavian journal of clinical and laboratory investigation · 2006
Relationship between PTEN gene expression and differentiation of human glioma.
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Yang Y, Shao N, Luo G, Li L, Nilsson-Ehle P, Xu N
Аннотация
OBJECTIVE: To investigate the relationship between PTEN gene expression and differentiation of glioma.
MATERIAL AND METHODS: The quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) method was applied to detect PTEN mRNA levels in glioma tissues. Tumor-adjacent normal tissues and benign brain tumors were used as controls. Relative PTEN mRNA levels were determined as the ratio of PTEN and GAPDH, which were correlated with the clinical-pathological results.
RESULTS: PTEN mRNA levels were significantly lower in the glioma tissues than in the benign brain tumors and tumor-adjacent normal tissues, whereas there were no statistical differences between benign brain tumor and the tumor-adjacent normal tissues. According to the pathological examinations, PTEN mRNA levels were higher in the high differential glioma than the low differential glioma.
CONCLUSIONS: PTEN gene expression was suppressed in the glioma, which is related to the clinical-pathological results. It is suggested that determination of PTEN mRNA levels by RT-PCR could be a novel marker of disease classification.
MATERIAL AND METHODS: The quantitative real-time reverse transcription-polymerase chain reaction (RT-PCR) method was applied to detect PTEN mRNA levels in glioma tissues. Tumor-adjacent normal tissues and benign brain tumors were used as controls. Relative PTEN mRNA levels were determined as the ratio of PTEN and GAPDH, which were correlated with the clinical-pathological results.
RESULTS: PTEN mRNA levels were significantly lower in the glioma tissues than in the benign brain tumors and tumor-adjacent normal tissues, whereas there were no statistical differences between benign brain tumor and the tumor-adjacent normal tissues. According to the pathological examinations, PTEN mRNA levels were higher in the high differential glioma than the low differential glioma.
CONCLUSIONS: PTEN gene expression was suppressed in the glioma, which is related to the clinical-pathological results. It is suggested that determination of PTEN mRNA levels by RT-PCR could be a novel marker of disease classification.
Предметные рубрики MeSH
AdolescentAdultAgedAstrocytomaBrain NeoplasmsChildFemaleGene ExpressionGenes, Tumor SuppressorGlioblastomaGliomaGlyceraldehyde-3-Phosphate DehydrogenasesHumansMaleMeningiomaMiddle AgedMutationNeuroma, AcousticPTEN PhosphohydrolaseRNA, MessengerRNA, NeoplasmReverse Transcriptase Polymerase Chain Reaction