Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology · 2008
Human bocavirus infection in children hospitalized with acute gastroenteritis in China.
СтатьяПоддержка исследования: вне правительства СШАНазвание и аннотация: Перевод в очереди
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Yu Jie-Mei, Li Dan-Di, Xu Zi-Qian, Cheng Wei-Xia, Zhang Qing, Li Hui-Ying, Cui Shu-Xian, Miao-Jin, Yang Su-Hua, Fang Zhao-Yin, Duan Zhao-Jun
Аннотация
BACKGROUND: Human bocavirus (HBoV) was first identified in children with acute respiratory-tract infections, but recent studies have revealed that HBoV is also frequently detected in fecal specimens from children with gastroenteritis.
OBJECTIVES: To investigate the prevalence of HBoV in children hospitalized with gastroenteritis in different areas of China.
STUDY DESIGN: Employing ELISA, RT-PCR or PCR, we evaluated 1216 fecal samples for common diarrheal agents from children aged less than 5-year-old hospitalized with acute gastroenteritis. MEGA software was used to construct phylogenetic trees of the VP1/VP2 partial sequences of the HBoV genome.
RESULTS: There were 67 HBoV-positive specimens, 52 (77.6%) were co-infected with rotavirus, norovirus, astrovirus, or enteric adenovirus. Statistical analysis of the clinical data indicated that children infected with both rotavirus and bocavirus did not have more severe clinical symptoms than children infected with rotavirus. The phylogenetic analysis of the VP1/VP2 partial sequences of the HBoV genome revealed a single genetic lineage.
CONCLUSIONS: Despite its high infection rate, there was no statistically significant a causual relationship between HBoV and gastroenteritis in children.
OBJECTIVES: To investigate the prevalence of HBoV in children hospitalized with gastroenteritis in different areas of China.
STUDY DESIGN: Employing ELISA, RT-PCR or PCR, we evaluated 1216 fecal samples for common diarrheal agents from children aged less than 5-year-old hospitalized with acute gastroenteritis. MEGA software was used to construct phylogenetic trees of the VP1/VP2 partial sequences of the HBoV genome.
RESULTS: There were 67 HBoV-positive specimens, 52 (77.6%) were co-infected with rotavirus, norovirus, astrovirus, or enteric adenovirus. Statistical analysis of the clinical data indicated that children infected with both rotavirus and bocavirus did not have more severe clinical symptoms than children infected with rotavirus. The phylogenetic analysis of the VP1/VP2 partial sequences of the HBoV genome revealed a single genetic lineage.
CONCLUSIONS: Despite its high infection rate, there was no statistically significant a causual relationship between HBoV and gastroenteritis in children.
Предметные рубрики MeSH
AdenoviridaeBocavirusChild, PreschoolChinaComorbidityEnzyme-Linked Immunosorbent AssayFecesFemaleGastroenteritisGenome, ViralHospitalizationHumansInfantInfant, NewbornMaleMolecular Sequence DataNorovirusParvoviridae InfectionsPhylogenyPolymerase Chain ReactionPrevalenceReverse Transcriptase Polymerase Chain ReactionRotavirusSequence Analysis, DNASequence HomologyViral Structural Proteins